The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation

Jing Mei Hsu, Jian Huang, Pamela B. Meluh, Brehon C. Laurent

Research output: Contribution to journalArticle

Abstract

The accurate segregation of chromosomes requires the kinetochore, a complex protein machine that assembles onto centromeric DNA to mediate attachment of replicated sister chromatids to the mitotic spindle apparatus. This study reveals an important role for the yeast RSC ATP-dependent chromatin-remodeling complex at the kinetochore in chromosome transmission. Mutations in genes encoding two core subunits of RSC, the ATPase Sth1p and the Snf5p homolog Sfh1p, interact genetically with mutations in genes encoding kinetochore proteins and with a mutation in centromeric DNA. RSC also interacts genetically and physically with the histone and histone variant components of centromeric chromatin. Importantly, RSC is localized to centromeric and centromere-proximal chromosomal regions, and its association with these loci is dependent on Sth1p. Both sth1 and sfh1 mutants exhibit altered centromeric and centromere-proximal chromatin structure and increased missegregation of authentic chromosomes. Finally, RSC is not required for centromeric deposition of the histone H3 variant Cse4p, suggesting that RSC plays a role in reconfiguring centromeric and flanking nucleosomes following Cse4p recruitment for proper chromosome transmission.

Original languageEnglish (US)
Pages (from-to)3202-3215
Number of pages14
JournalMolecular and Cellular Biology
Volume23
Issue number9
DOIs
StatePublished - May 2003
Externally publishedYes

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Kinetochores
Chromosome Segregation
Chromatin Assembly and Disassembly
Histones
Centromere
Chromosomes
Yeasts
Mutation
Chromatin
Spindle Apparatus
Chromatids
Nucleosomes
DNA
Genes
Adenosine Triphosphatases
Proteins
Adenosine Triphosphate

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation. / Hsu, Jing Mei; Huang, Jian; Meluh, Pamela B.; Laurent, Brehon C.

In: Molecular and Cellular Biology, Vol. 23, No. 9, 05.2003, p. 3202-3215.

Research output: Contribution to journalArticle

Hsu, Jing Mei ; Huang, Jian ; Meluh, Pamela B. ; Laurent, Brehon C. / The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation. In: Molecular and Cellular Biology. 2003 ; Vol. 23, No. 9. pp. 3202-3215.
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