The yeast endosomal Na+/H+ exchanger, Nhx1, confers osmotolerance following acute hypertonic shock

Richard Nass, Rajini Rao

Research output: Contribution to journalArticle

Abstract

Osmotolerance in yeast is regulated by at least two distinct mechanisms. The acquired response occurs following long-term exposure to hypertonic medium and requires the induction of the HOC-MAP (high-osmolarity glycerol mitogenactivated protein) kinase cascade to increase levels of the osmolyte glycerol. The acute response occurs following sudden exposure to high osmotica and appears to be dependent on normal vacuole function. In this study it is reported that the yeast endosomal/prevacuolar Na+/H+ exchanger Nhx1 contributes to osmotolerance following sudden exposure to hyperosmotic media. Vacuolar shrinkage and recovery in response to osmotic shock was altered in the Δnhx1 null mutant. Our results also show that the osmotolerance conferred by Nhx1 contributes to the postdiauxic/stationary-phase resistance to osmotic stress and allows for the continued growth of cells until the acquired osmotolerance response can occur.

Original languageEnglish (US)
Pages (from-to)3221-3228
Number of pages8
JournalMicrobiology
Volume145
Issue number11
StatePublished - Nov 1999

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Sodium-Hydrogen Antiporter
Osmotic Pressure
Glycerol
Yeasts
Vacuoles
Osmolar Concentration
Protein Kinases
Growth

Keywords

  • Hypertonic shock
  • Na/H exchanger
  • Osmotolerance
  • Saccharomyces cerevisiae
  • Vacuole

ASJC Scopus subject areas

  • Microbiology

Cite this

The yeast endosomal Na+/H+ exchanger, Nhx1, confers osmotolerance following acute hypertonic shock. / Nass, Richard; Rao, Rajini.

In: Microbiology, Vol. 145, No. 11, 11.1999, p. 3221-3228.

Research output: Contribution to journalArticle

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