The Wilson's disease protein expressed in Sf9 cells is phosphorylated

S. M. Vanderwerf, S. Lutsenko

Research output: Contribution to journalArticle

Abstract

The Wilson's disease protein (WNDP), a copper transporter, is a crucial mediator of copper homoeostasis in mammalian cells. We recently found that changes in copper concentration regulate the phosphorylation level of WNDP. WNDP phosphorylation was observed in several mammalian cell lines, suggesting that a common phosphorylation pathway exists in these cells. Here we demonstrate that WNDP expressed in Sf9 insect cells is also phosphorylated, as evidenced by metabolic labelling of these cells with [32P]Pi. Because the baculovirus system allows us to generate large amounts of protein, we are using this expression method to isolate WNDP and map the sites of WNDP phosphorylation. The identification of phosphorylation sites is the first step towards understanding the physiological role of WNDP phosphorylation.

Original languageEnglish (US)
Pages (from-to)739-741
Number of pages3
JournalBiochemical Society transactions
Volume30
Issue number4
DOIs
StatePublished - Aug 1 2002
Externally publishedYes

Keywords

  • Copper
  • Intracellular trafficking
  • Phosphorylation

ASJC Scopus subject areas

  • Biochemistry

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