TY - JOUR
T1 - The Whereabouts of microRNA Actions
T2 - Cytoplasm and Beyond
AU - Leung, Anthony K.L.
N1 - Funding Information:
The author would like to thank Phillip Sharp, Yoshinari Ando, Marriki Laiho, Sam Das, Jesse Zamudio, and Joe Fischer for critical reading of the manuscript, and Yun Wah Lam for discussing the current state of the field of nucleolar miRNAs. The miRNA research in the Leung lab is funded by the National Institutes of Health (NIH) grant R01-GM104135.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - MicroRNAs (miRNAs) are a conserved class of approximately 22 nucleotide (nt) short noncoding RNAs that normally silence gene expression via translational repression and/or degradation of targeted mRNAs in plants and animals. Identifying the whereabouts of miRNAs potentially informs miRNA functions, some of which are perhaps specialized to specific cellular compartments. In this review, the significance of miRNA localizations in the cytoplasm, including those at RNA granules and endomembranes, and the export of miRNAs to extracellular space will be discussed. How miRNA localizations and functions are regulated by protein modifications on the core miRNA-binding protein Argonaute (AGO) during normal and stress conditions will be explored, and in conclusion new AGO partners, non-AGO miRNA-binding proteins, and the emergent understanding of miRNAs found in the nucleoplasm, nucleoli, and mitochondria will be discussed. Mature miRNAs localize in multiple subcellular locations in the cytoplasm, such as RNA granules, endomembranes, and mitochondria, and are secreted out of cells via exosomes.Recent studies have revealed that mature miRNAs can also localize to the nucleus, where they could function in epigenetic regulation.The distributions of canonical and noncanonical forms of miRNA-induced silencing complexes suggest that different subcellular locations are required for the processing and degradation of miRNA itself, or for silencing or activation of miRNA targets.These subcellular distributions are differentially regulated by post-translational modifications as a function of cellular conditions, but one major question is whether such location-specific miRNAs are physiologically relevant.
AB - MicroRNAs (miRNAs) are a conserved class of approximately 22 nucleotide (nt) short noncoding RNAs that normally silence gene expression via translational repression and/or degradation of targeted mRNAs in plants and animals. Identifying the whereabouts of miRNAs potentially informs miRNA functions, some of which are perhaps specialized to specific cellular compartments. In this review, the significance of miRNA localizations in the cytoplasm, including those at RNA granules and endomembranes, and the export of miRNAs to extracellular space will be discussed. How miRNA localizations and functions are regulated by protein modifications on the core miRNA-binding protein Argonaute (AGO) during normal and stress conditions will be explored, and in conclusion new AGO partners, non-AGO miRNA-binding proteins, and the emergent understanding of miRNAs found in the nucleoplasm, nucleoli, and mitochondria will be discussed. Mature miRNAs localize in multiple subcellular locations in the cytoplasm, such as RNA granules, endomembranes, and mitochondria, and are secreted out of cells via exosomes.Recent studies have revealed that mature miRNAs can also localize to the nucleus, where they could function in epigenetic regulation.The distributions of canonical and noncanonical forms of miRNA-induced silencing complexes suggest that different subcellular locations are required for the processing and degradation of miRNA itself, or for silencing or activation of miRNA targets.These subcellular distributions are differentially regulated by post-translational modifications as a function of cellular conditions, but one major question is whether such location-specific miRNAs are physiologically relevant.
KW - Argonaute
KW - Circulating miRNAs
KW - Localization
KW - MiRNA
KW - Organelle
KW - Post-translational modification
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U2 - 10.1016/j.tcb.2015.07.005
DO - 10.1016/j.tcb.2015.07.005
M3 - Review article
C2 - 26410406
AN - SCOPUS:84942087859
SN - 0962-8924
VL - 25
SP - 601
EP - 610
JO - Trends in Cell Biology
JF - Trends in Cell Biology
IS - 10
M1 - 1164
ER -