The Vif accessory protein alters the cell cycle of human immunodeficiency virus type 1 infected cells

Jiangfang Wang, Jason M. Shackelford, Carolyn R. Casella, Debra K. Shivers, Eric L. Rapaport, Bindong Liu, Xiao Fang Yu, Terri H. Finkel

Research output: Contribution to journalArticle

Abstract

The viral infectivity factor gene (vif) of HIV-1 increases the infectivity of viral particles by inactivation of cellular anti-viral factors, and supports productive viral replication in primary human CD4 T cells and in certain non-permissive T cell lines. Here, we demonstrate that Vif also contributes to the arrest of HIV-1 infected cells in the G2 phase of the cell cycle. Viruses deleted in Vif or Vpr induce less cell cycle arrest than wild-type virus, while cells infected with HIV-1 deleted in both Vif and Vpr have a cell cycle profile equivalent to that of uninfected cells. Furthermore, expression of Vif alone induces accumulation of cells in the G2 phase of the cell cycle. These data demonstrate a novel role for Vif in cell cycle regulation and suggest that Vif and Vpr independently drive G2 arrest in HIV-1 infected cells. Our results may have implications for the actions and interactions of key HIV-1 accessory proteins in AIDS pathogenesis.

Original languageEnglish (US)
Pages (from-to)243-252
Number of pages10
JournalVirology
Volume359
Issue number2
DOIs
StatePublished - Mar 15 2007

Keywords

  • Cell cycle
  • HIV-1 accessory proteins
  • T cells
  • Vif
  • Vpr

ASJC Scopus subject areas

  • Virology

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  • Cite this

    Wang, J., Shackelford, J. M., Casella, C. R., Shivers, D. K., Rapaport, E. L., Liu, B., Yu, X. F., & Finkel, T. H. (2007). The Vif accessory protein alters the cell cycle of human immunodeficiency virus type 1 infected cells. Virology, 359(2), 243-252. https://doi.org/10.1016/j.virol.2006.09.026