The Vif accessory protein alters the cell cycle of human immunodeficiency virus type 1 infected cells

Jiangfang Wang, Jason M. Shackelford, Carolyn R. Casella, Debra K. Shivers, Eric L. Rapaport, Bindong Liu, Xiao Fang Yu, Terri H. Finkel

Research output: Contribution to journalArticle

Abstract

The viral infectivity factor gene (vif) of HIV-1 increases the infectivity of viral particles by inactivation of cellular anti-viral factors, and supports productive viral replication in primary human CD4 T cells and in certain non-permissive T cell lines. Here, we demonstrate that Vif also contributes to the arrest of HIV-1 infected cells in the G2 phase of the cell cycle. Viruses deleted in Vif or Vpr induce less cell cycle arrest than wild-type virus, while cells infected with HIV-1 deleted in both Vif and Vpr have a cell cycle profile equivalent to that of uninfected cells. Furthermore, expression of Vif alone induces accumulation of cells in the G2 phase of the cell cycle. These data demonstrate a novel role for Vif in cell cycle regulation and suggest that Vif and Vpr independently drive G2 arrest in HIV-1 infected cells. Our results may have implications for the actions and interactions of key HIV-1 accessory proteins in AIDS pathogenesis.

Original languageEnglish (US)
Pages (from-to)243-252
Number of pages10
JournalVirology
Volume359
Issue number2
DOIs
StatePublished - Mar 15 2007

Fingerprint

vif Gene Products
HIV-1
Cell Cycle
G2 Phase
Virus Inactivation
Viruses
T-Lymphocytes
Cell Cycle Checkpoints
Virion
Acquired Immunodeficiency Syndrome
Cell Line
Genes

Keywords

  • Cell cycle
  • HIV-1 accessory proteins
  • T cells
  • Vif
  • Vpr

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Wang, J., Shackelford, J. M., Casella, C. R., Shivers, D. K., Rapaport, E. L., Liu, B., ... Finkel, T. H. (2007). The Vif accessory protein alters the cell cycle of human immunodeficiency virus type 1 infected cells. Virology, 359(2), 243-252. https://doi.org/10.1016/j.virol.2006.09.026

The Vif accessory protein alters the cell cycle of human immunodeficiency virus type 1 infected cells. / Wang, Jiangfang; Shackelford, Jason M.; Casella, Carolyn R.; Shivers, Debra K.; Rapaport, Eric L.; Liu, Bindong; Yu, Xiao Fang; Finkel, Terri H.

In: Virology, Vol. 359, No. 2, 15.03.2007, p. 243-252.

Research output: Contribution to journalArticle

Wang, J, Shackelford, JM, Casella, CR, Shivers, DK, Rapaport, EL, Liu, B, Yu, XF & Finkel, TH 2007, 'The Vif accessory protein alters the cell cycle of human immunodeficiency virus type 1 infected cells', Virology, vol. 359, no. 2, pp. 243-252. https://doi.org/10.1016/j.virol.2006.09.026
Wang J, Shackelford JM, Casella CR, Shivers DK, Rapaport EL, Liu B et al. The Vif accessory protein alters the cell cycle of human immunodeficiency virus type 1 infected cells. Virology. 2007 Mar 15;359(2):243-252. https://doi.org/10.1016/j.virol.2006.09.026
Wang, Jiangfang ; Shackelford, Jason M. ; Casella, Carolyn R. ; Shivers, Debra K. ; Rapaport, Eric L. ; Liu, Bindong ; Yu, Xiao Fang ; Finkel, Terri H. / The Vif accessory protein alters the cell cycle of human immunodeficiency virus type 1 infected cells. In: Virology. 2007 ; Vol. 359, No. 2. pp. 243-252.
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