The vaginal microbiome and preterm birth

Jennifer M. Fettweis, Myrna G. Serrano, J. Paul Brooks, David J. Edwards, Philippe H. Girerd, Hardik I. Parikh, Bernice Huang, Tom J. Arodz, Laahirie Edupuganti, Abigail L. Glascock, Jie Xu, Nicole R. Jimenez, Stephany C. Vivadelli, Stephen S. Fong, Nihar U. Sheth, Sophonie Jean, Vladimir Lee, Yahya A. Bokhari, Ana M. Lara, Shreni D. MistryRobert A. Duckworth, Steven P. Bradley, Vishal N. Koparde, X. Valentine Orenda, Sarah H. Milton, Sarah K. Rozycki, Andrey V. Matveyev, Michelle L. Wright, Snehalata V. Huzurbazar, Eugenie M. Jackson, Ekaterina Smirnova, Jonas Korlach, Yu Chih Tsai, Molly R. Dickinson, Jamie L. Brooks, Jennifer I. Drake, Donald O. Chaffin, Amber L. Sexton, Michael G. Gravett, Craig E. Rubens, N. Romesh Wijesooriya, Karen D. Hendricks-Muñoz, Kimberly K. Jefferson, Jerome F. Strauss, Gregory A. Buck

Research output: Contribution to journalArticle

Abstract

The incidence of preterm birth exceeds 10% worldwide. There are significant disparities in the frequency of preterm birth among populations within countries, and women of African ancestry disproportionately bear the burden of risk in the United States. In the present study, we report a community resource that includes ‘omics’ data from approximately 12,000 samples as part of the integrative Human Microbiome Project. Longitudinal analyses of 16S ribosomal RNA, metagenomic, metatranscriptomic and cytokine profiles from 45 preterm and 90 term birth controls identified harbingers of preterm birth in this cohort of women predominantly of African ancestry. Women who delivered preterm exhibited significantly lower vaginal levels of Lactobacillus crispatus and higher levels of BVAB1, Sneathia amnii, TM7-H1, a group of Prevotella species and nine additional taxa. The first representative genomes of BVAB1 and TM7-H1 are described. Preterm-birth-associated taxa were correlated with proinflammatory cytokines in vaginal fluid. These findings highlight new opportunities for assessment of the risk of preterm birth.

Original languageEnglish (US)
Pages (from-to)1012-1021
Number of pages10
JournalNature medicine
Volume25
Issue number6
DOIs
StatePublished - Jun 1 2019
Externally publishedYes

Fingerprint

Microbiota
Premature Birth
16S Ribosomal RNA
Cytokines
Genes
Fluids
Prevotella
Term Birth
Metagenomics
Contraception
Genome
Incidence
Population

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Fettweis, J. M., Serrano, M. G., Brooks, J. P., Edwards, D. J., Girerd, P. H., Parikh, H. I., ... Buck, G. A. (2019). The vaginal microbiome and preterm birth. Nature medicine, 25(6), 1012-1021. https://doi.org/10.1038/s41591-019-0450-2

The vaginal microbiome and preterm birth. / Fettweis, Jennifer M.; Serrano, Myrna G.; Brooks, J. Paul; Edwards, David J.; Girerd, Philippe H.; Parikh, Hardik I.; Huang, Bernice; Arodz, Tom J.; Edupuganti, Laahirie; Glascock, Abigail L.; Xu, Jie; Jimenez, Nicole R.; Vivadelli, Stephany C.; Fong, Stephen S.; Sheth, Nihar U.; Jean, Sophonie; Lee, Vladimir; Bokhari, Yahya A.; Lara, Ana M.; Mistry, Shreni D.; Duckworth, Robert A.; Bradley, Steven P.; Koparde, Vishal N.; Orenda, X. Valentine; Milton, Sarah H.; Rozycki, Sarah K.; Matveyev, Andrey V.; Wright, Michelle L.; Huzurbazar, Snehalata V.; Jackson, Eugenie M.; Smirnova, Ekaterina; Korlach, Jonas; Tsai, Yu Chih; Dickinson, Molly R.; Brooks, Jamie L.; Drake, Jennifer I.; Chaffin, Donald O.; Sexton, Amber L.; Gravett, Michael G.; Rubens, Craig E.; Wijesooriya, N. Romesh; Hendricks-Muñoz, Karen D.; Jefferson, Kimberly K.; Strauss, Jerome F.; Buck, Gregory A.

In: Nature medicine, Vol. 25, No. 6, 01.06.2019, p. 1012-1021.

Research output: Contribution to journalArticle

Fettweis, JM, Serrano, MG, Brooks, JP, Edwards, DJ, Girerd, PH, Parikh, HI, Huang, B, Arodz, TJ, Edupuganti, L, Glascock, AL, Xu, J, Jimenez, NR, Vivadelli, SC, Fong, SS, Sheth, NU, Jean, S, Lee, V, Bokhari, YA, Lara, AM, Mistry, SD, Duckworth, RA, Bradley, SP, Koparde, VN, Orenda, XV, Milton, SH, Rozycki, SK, Matveyev, AV, Wright, ML, Huzurbazar, SV, Jackson, EM, Smirnova, E, Korlach, J, Tsai, YC, Dickinson, MR, Brooks, JL, Drake, JI, Chaffin, DO, Sexton, AL, Gravett, MG, Rubens, CE, Wijesooriya, NR, Hendricks-Muñoz, KD, Jefferson, KK, Strauss, JF & Buck, GA 2019, 'The vaginal microbiome and preterm birth', Nature medicine, vol. 25, no. 6, pp. 1012-1021. https://doi.org/10.1038/s41591-019-0450-2
Fettweis JM, Serrano MG, Brooks JP, Edwards DJ, Girerd PH, Parikh HI et al. The vaginal microbiome and preterm birth. Nature medicine. 2019 Jun 1;25(6):1012-1021. https://doi.org/10.1038/s41591-019-0450-2
Fettweis, Jennifer M. ; Serrano, Myrna G. ; Brooks, J. Paul ; Edwards, David J. ; Girerd, Philippe H. ; Parikh, Hardik I. ; Huang, Bernice ; Arodz, Tom J. ; Edupuganti, Laahirie ; Glascock, Abigail L. ; Xu, Jie ; Jimenez, Nicole R. ; Vivadelli, Stephany C. ; Fong, Stephen S. ; Sheth, Nihar U. ; Jean, Sophonie ; Lee, Vladimir ; Bokhari, Yahya A. ; Lara, Ana M. ; Mistry, Shreni D. ; Duckworth, Robert A. ; Bradley, Steven P. ; Koparde, Vishal N. ; Orenda, X. Valentine ; Milton, Sarah H. ; Rozycki, Sarah K. ; Matveyev, Andrey V. ; Wright, Michelle L. ; Huzurbazar, Snehalata V. ; Jackson, Eugenie M. ; Smirnova, Ekaterina ; Korlach, Jonas ; Tsai, Yu Chih ; Dickinson, Molly R. ; Brooks, Jamie L. ; Drake, Jennifer I. ; Chaffin, Donald O. ; Sexton, Amber L. ; Gravett, Michael G. ; Rubens, Craig E. ; Wijesooriya, N. Romesh ; Hendricks-Muñoz, Karen D. ; Jefferson, Kimberly K. ; Strauss, Jerome F. ; Buck, Gregory A. / The vaginal microbiome and preterm birth. In: Nature medicine. 2019 ; Vol. 25, No. 6. pp. 1012-1021.
@article{b3b4665896ae42b68e67250f3d3ab13a,
title = "The vaginal microbiome and preterm birth",
abstract = "The incidence of preterm birth exceeds 10{\%} worldwide. There are significant disparities in the frequency of preterm birth among populations within countries, and women of African ancestry disproportionately bear the burden of risk in the United States. In the present study, we report a community resource that includes ‘omics’ data from approximately 12,000 samples as part of the integrative Human Microbiome Project. Longitudinal analyses of 16S ribosomal RNA, metagenomic, metatranscriptomic and cytokine profiles from 45 preterm and 90 term birth controls identified harbingers of preterm birth in this cohort of women predominantly of African ancestry. Women who delivered preterm exhibited significantly lower vaginal levels of Lactobacillus crispatus and higher levels of BVAB1, Sneathia amnii, TM7-H1, a group of Prevotella species and nine additional taxa. The first representative genomes of BVAB1 and TM7-H1 are described. Preterm-birth-associated taxa were correlated with proinflammatory cytokines in vaginal fluid. These findings highlight new opportunities for assessment of the risk of preterm birth.",
author = "Fettweis, {Jennifer M.} and Serrano, {Myrna G.} and Brooks, {J. Paul} and Edwards, {David J.} and Girerd, {Philippe H.} and Parikh, {Hardik I.} and Bernice Huang and Arodz, {Tom J.} and Laahirie Edupuganti and Glascock, {Abigail L.} and Jie Xu and Jimenez, {Nicole R.} and Vivadelli, {Stephany C.} and Fong, {Stephen S.} and Sheth, {Nihar U.} and Sophonie Jean and Vladimir Lee and Bokhari, {Yahya A.} and Lara, {Ana M.} and Mistry, {Shreni D.} and Duckworth, {Robert A.} and Bradley, {Steven P.} and Koparde, {Vishal N.} and Orenda, {X. Valentine} and Milton, {Sarah H.} and Rozycki, {Sarah K.} and Matveyev, {Andrey V.} and Wright, {Michelle L.} and Huzurbazar, {Snehalata V.} and Jackson, {Eugenie M.} and Ekaterina Smirnova and Jonas Korlach and Tsai, {Yu Chih} and Dickinson, {Molly R.} and Brooks, {Jamie L.} and Drake, {Jennifer I.} and Chaffin, {Donald O.} and Sexton, {Amber L.} and Gravett, {Michael G.} and Rubens, {Craig E.} and Wijesooriya, {N. Romesh} and Hendricks-Mu{\~n}oz, {Karen D.} and Jefferson, {Kimberly K.} and Strauss, {Jerome F.} and Buck, {Gregory A.}",
year = "2019",
month = "6",
day = "1",
doi = "10.1038/s41591-019-0450-2",
language = "English (US)",
volume = "25",
pages = "1012--1021",
journal = "Nature Medicine",
issn = "1078-8956",
publisher = "Nature Publishing Group",
number = "6",

}

TY - JOUR

T1 - The vaginal microbiome and preterm birth

AU - Fettweis, Jennifer M.

AU - Serrano, Myrna G.

AU - Brooks, J. Paul

AU - Edwards, David J.

AU - Girerd, Philippe H.

AU - Parikh, Hardik I.

AU - Huang, Bernice

AU - Arodz, Tom J.

AU - Edupuganti, Laahirie

AU - Glascock, Abigail L.

AU - Xu, Jie

AU - Jimenez, Nicole R.

AU - Vivadelli, Stephany C.

AU - Fong, Stephen S.

AU - Sheth, Nihar U.

AU - Jean, Sophonie

AU - Lee, Vladimir

AU - Bokhari, Yahya A.

AU - Lara, Ana M.

AU - Mistry, Shreni D.

AU - Duckworth, Robert A.

AU - Bradley, Steven P.

AU - Koparde, Vishal N.

AU - Orenda, X. Valentine

AU - Milton, Sarah H.

AU - Rozycki, Sarah K.

AU - Matveyev, Andrey V.

AU - Wright, Michelle L.

AU - Huzurbazar, Snehalata V.

AU - Jackson, Eugenie M.

AU - Smirnova, Ekaterina

AU - Korlach, Jonas

AU - Tsai, Yu Chih

AU - Dickinson, Molly R.

AU - Brooks, Jamie L.

AU - Drake, Jennifer I.

AU - Chaffin, Donald O.

AU - Sexton, Amber L.

AU - Gravett, Michael G.

AU - Rubens, Craig E.

AU - Wijesooriya, N. Romesh

AU - Hendricks-Muñoz, Karen D.

AU - Jefferson, Kimberly K.

AU - Strauss, Jerome F.

AU - Buck, Gregory A.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - The incidence of preterm birth exceeds 10% worldwide. There are significant disparities in the frequency of preterm birth among populations within countries, and women of African ancestry disproportionately bear the burden of risk in the United States. In the present study, we report a community resource that includes ‘omics’ data from approximately 12,000 samples as part of the integrative Human Microbiome Project. Longitudinal analyses of 16S ribosomal RNA, metagenomic, metatranscriptomic and cytokine profiles from 45 preterm and 90 term birth controls identified harbingers of preterm birth in this cohort of women predominantly of African ancestry. Women who delivered preterm exhibited significantly lower vaginal levels of Lactobacillus crispatus and higher levels of BVAB1, Sneathia amnii, TM7-H1, a group of Prevotella species and nine additional taxa. The first representative genomes of BVAB1 and TM7-H1 are described. Preterm-birth-associated taxa were correlated with proinflammatory cytokines in vaginal fluid. These findings highlight new opportunities for assessment of the risk of preterm birth.

AB - The incidence of preterm birth exceeds 10% worldwide. There are significant disparities in the frequency of preterm birth among populations within countries, and women of African ancestry disproportionately bear the burden of risk in the United States. In the present study, we report a community resource that includes ‘omics’ data from approximately 12,000 samples as part of the integrative Human Microbiome Project. Longitudinal analyses of 16S ribosomal RNA, metagenomic, metatranscriptomic and cytokine profiles from 45 preterm and 90 term birth controls identified harbingers of preterm birth in this cohort of women predominantly of African ancestry. Women who delivered preterm exhibited significantly lower vaginal levels of Lactobacillus crispatus and higher levels of BVAB1, Sneathia amnii, TM7-H1, a group of Prevotella species and nine additional taxa. The first representative genomes of BVAB1 and TM7-H1 are described. Preterm-birth-associated taxa were correlated with proinflammatory cytokines in vaginal fluid. These findings highlight new opportunities for assessment of the risk of preterm birth.

UR - http://www.scopus.com/inward/record.url?scp=85066501802&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85066501802&partnerID=8YFLogxK

U2 - 10.1038/s41591-019-0450-2

DO - 10.1038/s41591-019-0450-2

M3 - Article

C2 - 31142849

AN - SCOPUS:85066501802

VL - 25

SP - 1012

EP - 1021

JO - Nature Medicine

JF - Nature Medicine

SN - 1078-8956

IS - 6

ER -