The usefulness of CD71 expression by flow cytometry for differentiating indolent from aggressive CD10+ B-cell lymphomas

Julie M. Wu, Michael J. Borowitz, Edward G. Weir

Research output: Contribution to journalArticle

Abstract

We studied 34 low- and 30 high-grade CD10+ B-cell lymphomas. Forward light scatter (FSC) and CD71 fluorescence intensity (CD71i) of tumor cells were measured and normalized by corresponding values for resting T cells. Significant differences in CD71i values between low- and high-grade lymphomas were observed by the Mann-Whitney U test (P < .007) and receiver operating characteristic (ROC) curve analysis (P < .001). FSC was not significantly different between low- and high-grade lymphomas; the area under the ROC curve was less than that for CD71i. Neither FSC nor CD71i significantly differentiated follicular lymphoma (FL) grades. A comparison of all FLs (grades 1-3) and non-FL high-grade lymphomas (Burkitt lymphoma [BL] and large B-cell lymphoma [LBCL]) showed significant differences in CD71i (P < .001) and FSC (P = .021). Differences were significant in CD71i and FSC between FL and LBCL (P < .007) but not between FL and BL. CD71i is more potent than FSC for distinguishing CD10+ low-from high-grade lymphomas and FL from non-FL high-grade lymphomas. Sensitivity and specificity are limited owing to inability to identify FL3. In ROC analysis, a high value for CD71i can identify BL and LBCL with a high degree of certainty.

Original languageEnglish (US)
Pages (from-to)39-46
Number of pages8
JournalAmerican journal of clinical pathology
Volume126
Issue number1
DOIs
StatePublished - Jul 2006

Keywords

  • B-cell lymphoma
  • CD71
  • Flow cytometry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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