The Use of Thin-Layer Chromatography on Poly(ethyleneimine)cellulose to Facilitate Assays of ATP-ADP Exchange, ATP-Pi Exchange, Adenylate Kinase, and Nucleoside Diphosphokinase Activity

Peter L. Pedersen; William A. Catterall

doi:10.1016/0076-6879(79)55032-0

The Use of Thin-Layer Chromatography on Poly(ethyleneimine)cellulose to Facilitate Assays of ATP-ADP Exchange, ATP-P_i Exchange, Adenylate Kinase, and Nucleoside Diphosphokinase Activity

Peter L. Pedersen, William A. Catterall

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The technique of thin-layer chromatography on PEI-cellulose is useful in facilitating radiactive assay of ATP-ADP exchange, ATP-P_i exchange, adenylate kinase, and nucleoside disphosphokinase activities of mitochondria. In all assays the technique is used to separate nucleotide components after the enzymatic reaction has been terminated. The technique is also used to separate P_i from nucleotides. Solvent systems that are useful in the phosphotransferase assays described here are summarized in Table I.

Original language	English (US)
Pages (from-to)	283-289
Number of pages	7
Journal	Methods in enzymology
Volume	55
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(79)55032-0
State	Published - Jan 1 1979
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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abstract = "The technique of thin-layer chromatography on PEI-cellulose is useful in facilitating radiactive assay of ATP-ADP exchange, ATP-Pi exchange, adenylate kinase, and nucleoside disphosphokinase activities of mitochondria. In all assays the technique is used to separate nucleotide components after the enzymatic reaction has been terminated. The technique is also used to separate Pi from nucleotides. Solvent systems that are useful in the phosphotransferase assays described here are summarized in Table I.",

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AU - Catterall, William A.

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N2 - The technique of thin-layer chromatography on PEI-cellulose is useful in facilitating radiactive assay of ATP-ADP exchange, ATP-Pi exchange, adenylate kinase, and nucleoside disphosphokinase activities of mitochondria. In all assays the technique is used to separate nucleotide components after the enzymatic reaction has been terminated. The technique is also used to separate Pi from nucleotides. Solvent systems that are useful in the phosphotransferase assays described here are summarized in Table I.

AB - The technique of thin-layer chromatography on PEI-cellulose is useful in facilitating radiactive assay of ATP-ADP exchange, ATP-Pi exchange, adenylate kinase, and nucleoside disphosphokinase activities of mitochondria. In all assays the technique is used to separate nucleotide components after the enzymatic reaction has been terminated. The technique is also used to separate Pi from nucleotides. Solvent systems that are useful in the phosphotransferase assays described here are summarized in Table I.

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The Use of Thin-Layer Chromatography on Poly(ethyleneimine)cellulose to Facilitate Assays of ATP-ADP Exchange, ATP-P_i Exchange, Adenylate Kinase, and Nucleoside Diphosphokinase Activity

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