The Use of Thin-Layer Chromatography on Poly(ethyleneimine)cellulose to Facilitate Assays of ATP-ADP Exchange, ATP-Pi Exchange, Adenylate Kinase, and Nucleoside Diphosphokinase Activity

Peter L. Pedersen, William A. Catterall

Research output: Contribution to journalArticlepeer-review

Abstract

The technique of thin-layer chromatography on PEI-cellulose is useful in facilitating radiactive assay of ATP-ADP exchange, ATP-Pi exchange, adenylate kinase, and nucleoside disphosphokinase activities of mitochondria. In all assays the technique is used to separate nucleotide components after the enzymatic reaction has been terminated. The technique is also used to separate Pi from nucleotides. Solvent systems that are useful in the phosphotransferase assays described here are summarized in Table I.

Original languageEnglish (US)
Pages (from-to)283-289
Number of pages7
JournalMethods in enzymology
Volume55
Issue numberC
DOIs
StatePublished - Jan 1 1979

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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