Synthetic peptides have been used successfully as probes in studying the relationship between protein structure and function. The biological and functionally important regions of three muscle proteins, troponin C, troponin I, and actin, are presented in this review. The small size of these peptides has enabled the determination of their free or bound structures by two-dimensional or trNOE NMR techniques. These biological and structural results have greatly advanced our understanding of the complex protein-protein interactions involved in muscle regulation. These studies emphasize that small synthetic peptides can mimic the biological activity of their native protein and that small linear sequences are important molecular recognition sites in protein-protein interactions. In addition, three-dimensional structural information is still inherent in the amino acid sequence of these peptides.
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)