The use of restriction fragment polymorphisms to identify the cell line MCF-7

Kathryn A. Graham, Jeffrey M. Trent, C. Kent Osborne, Charles M. McGrath, Mark D. Minden, Ronald N. Buick

Research output: Contribution to journalArticle

Abstract

We have recently documented variations in the level of N-ras gene amplification in independently passaged MCF-7 cells. To establish if these differences are due to a lack of genetic identity between current MCF-7 passages and the original cell line, we have performed karyological and restriction fragment length polymorphism (RFLP) analyses. Documentation of a combination of restriction fragment patterns which are characteristic of MCF-7 has been facilitated by an analysis of DNA from an early-passage of MCF-7 from the Michigan Cancer Foundation (MCF). In addition, such early passage cells and other MCF-7 lines also share a unique amplification of the N-ras oncogene. Using these criteria and karyotypic analysis it can be shown that a sample of MCF-7 obtained from the American Type Culture Collection (-ATCC) was derived from a different individual than was the original MCF-7 cell line. It is important that researchers verify the relationship of current cell lines to the original MCF-7. Furthermore, the techniques described here provide a powerful tool which may be used to assess the identity of cell stocks.

Original languageEnglish (US)
Pages (from-to)29-34
Number of pages6
JournalBreast Cancer Research and Treatment
Volume8
Issue number1
DOIs
StatePublished - Feb 1986
Externally publishedYes

Fingerprint

Cell Line
MCF-7 Cells
Neoplasms
ras Genes
Gene Amplification
Pedigree
Restriction Fragment Length Polymorphisms
Documentation
Research Personnel
DNA

Keywords

  • cell line identity
  • MCF-7
  • N-ras gene amplification
  • restriction fragment length polymorphism

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Graham, K. A., Trent, J. M., Osborne, C. K., McGrath, C. M., Minden, M. D., & Buick, R. N. (1986). The use of restriction fragment polymorphisms to identify the cell line MCF-7. Breast Cancer Research and Treatment, 8(1), 29-34. https://doi.org/10.1007/BF01805922

The use of restriction fragment polymorphisms to identify the cell line MCF-7. / Graham, Kathryn A.; Trent, Jeffrey M.; Osborne, C. Kent; McGrath, Charles M.; Minden, Mark D.; Buick, Ronald N.

In: Breast Cancer Research and Treatment, Vol. 8, No. 1, 02.1986, p. 29-34.

Research output: Contribution to journalArticle

Graham, KA, Trent, JM, Osborne, CK, McGrath, CM, Minden, MD & Buick, RN 1986, 'The use of restriction fragment polymorphisms to identify the cell line MCF-7', Breast Cancer Research and Treatment, vol. 8, no. 1, pp. 29-34. https://doi.org/10.1007/BF01805922
Graham, Kathryn A. ; Trent, Jeffrey M. ; Osborne, C. Kent ; McGrath, Charles M. ; Minden, Mark D. ; Buick, Ronald N. / The use of restriction fragment polymorphisms to identify the cell line MCF-7. In: Breast Cancer Research and Treatment. 1986 ; Vol. 8, No. 1. pp. 29-34.
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