The use of immunoprecipitation to study the synthesis and cleavage processing of viral proteins

Stuart Z. Shapiro, J. Thomas August

Research output: Contribution to journalArticle

Abstract

Several factors have been considered in developing an immunoprecipitation procedure which minimizes the background of non-immunospecifically precipitated protein. The procedure evolved utilizes the technique of direct immunoprecipitation (specific antibody plus sufficient unlabeled antigen to form a precipitable complex). High speed centrifugation of the cell extract immediately prior to immunoprecipitation and the use of test tubes coated with silicone greatly reduced background precipitation.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalJournal of Immunological Methods
Volume13
Issue number2
DOIs
StatePublished - Nov 1976

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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