The use of immunoprecipitation to study the synthesis and cleavage processing of viral proteins

Stuart Z. Shapiro; J. Thomas August

doi:10.1016/0022-1759(76)90153-8

The use of immunoprecipitation to study the synthesis and cleavage processing of viral proteins

Stuart Z. Shapiro, J. Thomas August

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Several factors have been considered in developing an immunoprecipitation procedure which minimizes the background of non-immunospecifically precipitated protein. The procedure evolved utilizes the technique of direct immunoprecipitation (specific antibody plus sufficient unlabeled antigen to form a precipitable complex). High speed centrifugation of the cell extract immediately prior to immunoprecipitation and the use of test tubes coated with silicone greatly reduced background precipitation.

Original language	English (US)
Pages (from-to)	153-159
Number of pages	7
Journal	Journal of Immunological Methods
Volume	13
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(76)90153-8
State	Published - Nov 1976
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(76)90153-8

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title = "The use of immunoprecipitation to study the synthesis and cleavage processing of viral proteins",

abstract = "Several factors have been considered in developing an immunoprecipitation procedure which minimizes the background of non-immunospecifically precipitated protein. The procedure evolved utilizes the technique of direct immunoprecipitation (specific antibody plus sufficient unlabeled antigen to form a precipitable complex). High speed centrifugation of the cell extract immediately prior to immunoprecipitation and the use of test tubes coated with silicone greatly reduced background precipitation.",

author = "Shapiro, {Stuart Z.} and August, {J. Thomas}",

note = "Funding Information: In theory, the technique of immunoprecipitation separates specific proteins from the huge background of other newly synthesized protein in the * This study was supported by NIH Training Grant Number 5T5 GM 1674 and Public Health Service Grant GM 11301-11 from the National Institute of General Medical Sciences and by Public I'~ealth Service Contract 7\]-2251 within the Virus Cancer Program of the National Cancer Institute.",

year = "1976",

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doi = "10.1016/0022-1759(76)90153-8",

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AU - Shapiro, Stuart Z.

AU - August, J. Thomas

N1 - Funding Information: In theory, the technique of immunoprecipitation separates specific proteins from the huge background of other newly synthesized protein in the * This study was supported by NIH Training Grant Number 5T5 GM 1674 and Public Health Service Grant GM 11301-11 from the National Institute of General Medical Sciences and by Public I'~ealth Service Contract 7\]-2251 within the Virus Cancer Program of the National Cancer Institute.

PY - 1976/11

Y1 - 1976/11

N2 - Several factors have been considered in developing an immunoprecipitation procedure which minimizes the background of non-immunospecifically precipitated protein. The procedure evolved utilizes the technique of direct immunoprecipitation (specific antibody plus sufficient unlabeled antigen to form a precipitable complex). High speed centrifugation of the cell extract immediately prior to immunoprecipitation and the use of test tubes coated with silicone greatly reduced background precipitation.

AB - Several factors have been considered in developing an immunoprecipitation procedure which minimizes the background of non-immunospecifically precipitated protein. The procedure evolved utilizes the technique of direct immunoprecipitation (specific antibody plus sufficient unlabeled antigen to form a precipitable complex). High speed centrifugation of the cell extract immediately prior to immunoprecipitation and the use of test tubes coated with silicone greatly reduced background precipitation.

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ER -

The use of immunoprecipitation to study the synthesis and cleavage processing of viral proteins

Abstract

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