The use of hybridoma antibodies to probe the antigenic determinants of thyroglobulin.

N. R. Rose, M. Accavitti, E. F. Pydyn, M. A. Leon, R. K. Brown

Research output: Contribution to journalArticle

Abstract

Using monoclonal antibodies we have begun to define the epitopes of the murine thyroglobulin molecule that elicit autoimmune responses. Based on the principle of complementation, 18 monoclonal antibodies were classified into 8 groups, defined by their reactions with the same or neighboring determinants. Further distinctions between the monoclonals were drawn from comparisons of their cross-reactions with thyroglobulins of other species and their patterns in isoelectric focusing. One low molecular weight fragment of bovine thyroglobulin, which cross-reacts extensively with thyroglobulins of other species, has been isolated and partially characterized.

Original languageEnglish (US)
Pages (from-to)23-35
Number of pages13
JournalAdvances in Experimental Medicine and Biology
Volume150
StatePublished - 1982
Externally publishedYes

Fingerprint

Thyroglobulin
Hybridomas
Epitopes
Antibodies
Monoclonal Antibodies
Cross Reactions
Isoelectric Focusing
Autoimmunity
Molecular Weight
Molecular weight
Molecules

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Rose, N. R., Accavitti, M., Pydyn, E. F., Leon, M. A., & Brown, R. K. (1982). The use of hybridoma antibodies to probe the antigenic determinants of thyroglobulin. Advances in Experimental Medicine and Biology, 150, 23-35.

The use of hybridoma antibodies to probe the antigenic determinants of thyroglobulin. / Rose, N. R.; Accavitti, M.; Pydyn, E. F.; Leon, M. A.; Brown, R. K.

In: Advances in Experimental Medicine and Biology, Vol. 150, 1982, p. 23-35.

Research output: Contribution to journalArticle

Rose, NR, Accavitti, M, Pydyn, EF, Leon, MA & Brown, RK 1982, 'The use of hybridoma antibodies to probe the antigenic determinants of thyroglobulin.', Advances in Experimental Medicine and Biology, vol. 150, pp. 23-35.
Rose, N. R. ; Accavitti, M. ; Pydyn, E. F. ; Leon, M. A. ; Brown, R. K. / The use of hybridoma antibodies to probe the antigenic determinants of thyroglobulin. In: Advances in Experimental Medicine and Biology. 1982 ; Vol. 150. pp. 23-35.
@article{62fc47f9ae934faf81e72246abc5ecb0,
title = "The use of hybridoma antibodies to probe the antigenic determinants of thyroglobulin.",
abstract = "Using monoclonal antibodies we have begun to define the epitopes of the murine thyroglobulin molecule that elicit autoimmune responses. Based on the principle of complementation, 18 monoclonal antibodies were classified into 8 groups, defined by their reactions with the same or neighboring determinants. Further distinctions between the monoclonals were drawn from comparisons of their cross-reactions with thyroglobulins of other species and their patterns in isoelectric focusing. One low molecular weight fragment of bovine thyroglobulin, which cross-reacts extensively with thyroglobulins of other species, has been isolated and partially characterized.",
author = "Rose, {N. R.} and M. Accavitti and Pydyn, {E. F.} and Leon, {M. A.} and Brown, {R. K.}",
year = "1982",
language = "English (US)",
volume = "150",
pages = "23--35",
journal = "Advances in Experimental Medicine and Biology",
issn = "0065-2598",
publisher = "Springer New York",

}

TY - JOUR

T1 - The use of hybridoma antibodies to probe the antigenic determinants of thyroglobulin.

AU - Rose, N. R.

AU - Accavitti, M.

AU - Pydyn, E. F.

AU - Leon, M. A.

AU - Brown, R. K.

PY - 1982

Y1 - 1982

N2 - Using monoclonal antibodies we have begun to define the epitopes of the murine thyroglobulin molecule that elicit autoimmune responses. Based on the principle of complementation, 18 monoclonal antibodies were classified into 8 groups, defined by their reactions with the same or neighboring determinants. Further distinctions between the monoclonals were drawn from comparisons of their cross-reactions with thyroglobulins of other species and their patterns in isoelectric focusing. One low molecular weight fragment of bovine thyroglobulin, which cross-reacts extensively with thyroglobulins of other species, has been isolated and partially characterized.

AB - Using monoclonal antibodies we have begun to define the epitopes of the murine thyroglobulin molecule that elicit autoimmune responses. Based on the principle of complementation, 18 monoclonal antibodies were classified into 8 groups, defined by their reactions with the same or neighboring determinants. Further distinctions between the monoclonals were drawn from comparisons of their cross-reactions with thyroglobulins of other species and their patterns in isoelectric focusing. One low molecular weight fragment of bovine thyroglobulin, which cross-reacts extensively with thyroglobulins of other species, has been isolated and partially characterized.

UR - http://www.scopus.com/inward/record.url?scp=0020392623&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020392623&partnerID=8YFLogxK

M3 - Article

C2 - 6183944

AN - SCOPUS:0020392623

VL - 150

SP - 23

EP - 35

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

SN - 0065-2598

ER -