The use of high-pressure liquid chromatography in the preparation of tritium-labeled chloramphenicol and its analogs

Jackie L. Martin, Anne M. Taburet, Lance R. Pohl

Research output: Contribution to journalArticle

Abstract

The 1-hydroxy epimers of chloramphenicol and thiamphenicol formed from the reduction of the respective 1-oxo derivatives with [3H]NaBH4 have been separated preparatively by high-pressure liquid chromatography on a μBondapak C18 column. This separation procedure permits the facile and rapid preparation of the 1-3H-labeled derivatives of chloramphenicol and its analogs.

Original languageEnglish (US)
Pages (from-to)215-219
Number of pages5
JournalAnalytical biochemistry
Volume96
Issue number1
DOIs
StatePublished - Jul 1 1979

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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