The use of glycol methacrylate embedding for studies of chromatin distribution and fine structure in salmon spermatid nuclei

Barry R. Zirkin

doi:10.1007/BF00494075

The use of glycol methacrylate embedding for studies of chromatin distribution and fine structure in salmon spermatid nuclei

Barry R. Zirkin

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Glycol methacrylate has been used as an embedding medium for studies of spermiogenesis in the salmon. DNA and basic proteins are shown to stain with the same specificity in thick (0.5-1.0 μ) sections of GMA-embedded salmon testes as in sections of comparably fixed, paraffin-embedded testes. Stain can be localized far more precisely in GMA sections than in paraffin sections due to the thinness of the sections and to the excellent structural preservation of nuclei. In addition, ultra-thin sections of GMA-embedded salmon testes can be observed with the electron microscope, and this permits exact correlation between nuclear fine structure and chemical composition in consecutive sections of the same nuclei.

Original language	English (US)
Pages (from-to)	137-146
Number of pages	10
Journal	Histochemistry
Volume	44
Issue number	2
DOIs	https://doi.org/10.1007/BF00494075
State	Published - Jun 1975
Externally published	Yes

ASJC Scopus subject areas

Anatomy
General Agricultural and Biological Sciences

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10.1007/BF00494075

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abstract = "Glycol methacrylate has been used as an embedding medium for studies of spermiogenesis in the salmon. DNA and basic proteins are shown to stain with the same specificity in thick (0.5-1.0 μ) sections of GMA-embedded salmon testes as in sections of comparably fixed, paraffin-embedded testes. Stain can be localized far more precisely in GMA sections than in paraffin sections due to the thinness of the sections and to the excellent structural preservation of nuclei. In addition, ultra-thin sections of GMA-embedded salmon testes can be observed with the electron microscope, and this permits exact correlation between nuclear fine structure and chemical composition in consecutive sections of the same nuclei.",

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AU - Zirkin, Barry R.

PY - 1975/6

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N2 - Glycol methacrylate has been used as an embedding medium for studies of spermiogenesis in the salmon. DNA and basic proteins are shown to stain with the same specificity in thick (0.5-1.0 μ) sections of GMA-embedded salmon testes as in sections of comparably fixed, paraffin-embedded testes. Stain can be localized far more precisely in GMA sections than in paraffin sections due to the thinness of the sections and to the excellent structural preservation of nuclei. In addition, ultra-thin sections of GMA-embedded salmon testes can be observed with the electron microscope, and this permits exact correlation between nuclear fine structure and chemical composition in consecutive sections of the same nuclei.

AB - Glycol methacrylate has been used as an embedding medium for studies of spermiogenesis in the salmon. DNA and basic proteins are shown to stain with the same specificity in thick (0.5-1.0 μ) sections of GMA-embedded salmon testes as in sections of comparably fixed, paraffin-embedded testes. Stain can be localized far more precisely in GMA sections than in paraffin sections due to the thinness of the sections and to the excellent structural preservation of nuclei. In addition, ultra-thin sections of GMA-embedded salmon testes can be observed with the electron microscope, and this permits exact correlation between nuclear fine structure and chemical composition in consecutive sections of the same nuclei.

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ER -

The use of glycol methacrylate embedding for studies of chromatin distribution and fine structure in salmon spermatid nuclei

Abstract

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