The use of genetically modified embryonic stem cells to generate transgenic mice

M. V. Pryzhkova; I. R. Zakeeva; A. V. Kibardin; G. P. Georgiev; S. L. Kiselev

doi:10.1023/B:RUGE.0000021621.36984.5c

The use of genetically modified embryonic stem cells to generate transgenic mice

M. V. Pryzhkova, I. R. Zakeeva, A. V. Kibardin, G. P. Georgiev, S. L. Kiselev

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Targeted genetic modification of embryonic stem cells (ESC) was used to obtain nondifferentiated cell clones containing the foreign genetic material in the genome. It was demonstrated that transgenic animals may be obtained by ESC injection in preimplantation embryos and subsequent transplantation of the embryos into a recipient female. Using this method, we constructed chimeric animals with a modified genome.

Original language	English (US)
Pages (from-to)	235-238
Number of pages	4
Journal	Russian Journal of Genetics
Volume	40
Issue number	3
DOIs	https://doi.org/10.1023/B:RUGE.0000021621.36984.5c
State	Published - Mar 2004
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "The use of genetically modified embryonic stem cells to generate transgenic mice",

abstract = "Targeted genetic modification of embryonic stem cells (ESC) was used to obtain nondifferentiated cell clones containing the foreign genetic material in the genome. It was demonstrated that transgenic animals may be obtained by ESC injection in preimplantation embryos and subsequent transplantation of the embryos into a recipient female. Using this method, we constructed chimeric animals with a modified genome.",

author = "Pryzhkova, {M. V.} and Zakeeva, {I. R.} and Kibardin, {A. V.} and Georgiev, {G. P.} and Kiselev, {S. L.}",

note = "Funding Information: This work was supported by the Russian Foundation for Basic Research (project no. 02-04-48568), the Moscow Oncological Program, and the Moscow Committee for Science and Technology. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",

year = "2004",

month = mar,

doi = "10.1023/B:RUGE.0000021621.36984.5c",

language = "English (US)",

volume = "40",

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AU - Pryzhkova, M. V.

AU - Zakeeva, I. R.

AU - Kibardin, A. V.

AU - Georgiev, G. P.

AU - Kiselev, S. L.

N1 - Funding Information: This work was supported by the Russian Foundation for Basic Research (project no. 02-04-48568), the Moscow Oncological Program, and the Moscow Committee for Science and Technology. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2004/3

Y1 - 2004/3

N2 - Targeted genetic modification of embryonic stem cells (ESC) was used to obtain nondifferentiated cell clones containing the foreign genetic material in the genome. It was demonstrated that transgenic animals may be obtained by ESC injection in preimplantation embryos and subsequent transplantation of the embryos into a recipient female. Using this method, we constructed chimeric animals with a modified genome.

AB - Targeted genetic modification of embryonic stem cells (ESC) was used to obtain nondifferentiated cell clones containing the foreign genetic material in the genome. It was demonstrated that transgenic animals may be obtained by ESC injection in preimplantation embryos and subsequent transplantation of the embryos into a recipient female. Using this method, we constructed chimeric animals with a modified genome.

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The use of genetically modified embryonic stem cells to generate transgenic mice

Abstract

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