The use of confocal microscopy in evaluating corneal wound healing after excimer laser keratectomy

J. P. Essepian, R. K. Rajpal, D. T. Azar, K. New, R. Antonacci, W. Shields, W. J. Stark

Research output: Contribution to journalArticle

Abstract

Corneal wound healing following excimer laser keratectomy is the major cause of regression of treatment results. The amount of anterior stromal haze that develops may be influenced by topical medications. Over a period of 6 months, we followed 15 New Zealand white rabbit eyes that underwent excimer laser keratectomy with the VISX 193-nm ArF laser at a fluence of 150 mJ/cm2 for a depth of 130 μm. Eyes were randomized to treatment with prednisolone acetate, diclofenac sodium (Voltaren), a combination of both, and a control group. Drops were administered four times a day for 1 week, two times a day for 3 weeks, and the drops were then tapered. All eyes were reepithelialized by 5 to 7 days. The tandem scanning confocal microscope (TSCM) was used to evaluate the corneal wound in vivo weekly for a month and monthly for 6 months. During the early postoperative period, the TSCM revealed significant anterior stromal keratocyte activation with cell elongation and the spindle- shaped appearance of fibroblasts in all groups. Collagenous stromal scarring was evident initially, then slowly decreased in all treatment groups. This study shows that TSCM is clinically useful for successive in vivo examinations of corneal wounds after excimer laser keratectomy and for comparing the effects of various topical medications.

Original languageEnglish (US)
Pages (from-to)300-304
Number of pages5
JournalScanning
Volume16
Issue number5
StatePublished - Sep 1994

Fingerprint

wound healing
Confocal microscopy
Excimer lasers
excimer lasers
Microscopes
microscopes
microscopy
Scanning
scanning
haze
spindles
rabbits
New Zealand
fibroblasts
Fibroblasts
elongation
regression analysis
Elongation
acetates
fluence

Keywords

  • corneal wound healing
  • excimer laser
  • haze
  • tandem scanning confocal microscopy

ASJC Scopus subject areas

  • Instrumentation

Cite this

Essepian, J. P., Rajpal, R. K., Azar, D. T., New, K., Antonacci, R., Shields, W., & Stark, W. J. (1994). The use of confocal microscopy in evaluating corneal wound healing after excimer laser keratectomy. Scanning, 16(5), 300-304.

The use of confocal microscopy in evaluating corneal wound healing after excimer laser keratectomy. / Essepian, J. P.; Rajpal, R. K.; Azar, D. T.; New, K.; Antonacci, R.; Shields, W.; Stark, W. J.

In: Scanning, Vol. 16, No. 5, 09.1994, p. 300-304.

Research output: Contribution to journalArticle

Essepian, JP, Rajpal, RK, Azar, DT, New, K, Antonacci, R, Shields, W & Stark, WJ 1994, 'The use of confocal microscopy in evaluating corneal wound healing after excimer laser keratectomy', Scanning, vol. 16, no. 5, pp. 300-304.
Essepian JP, Rajpal RK, Azar DT, New K, Antonacci R, Shields W et al. The use of confocal microscopy in evaluating corneal wound healing after excimer laser keratectomy. Scanning. 1994 Sep;16(5):300-304.
Essepian, J. P. ; Rajpal, R. K. ; Azar, D. T. ; New, K. ; Antonacci, R. ; Shields, W. ; Stark, W. J. / The use of confocal microscopy in evaluating corneal wound healing after excimer laser keratectomy. In: Scanning. 1994 ; Vol. 16, No. 5. pp. 300-304.
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