The urinary excretion of nucleosides of ribonucleic acid by patients with advanced cancer

T. Phillip Waalkes; Charles W. Gehrke; Robert W. Zumwalt; Sai Y. Chang; Duane B. Lakings; Douglass C. Tormey; David L. Ahmann; Charles G. Moertel

doi:10.1002/1097-0142(197508)36:2<390::AID-CNCR2820360214>3.0.CO;2-C

The urinary excretion of nucleosides of ribonucleic acid by patients with advanced cancer

T. Phillip Waalkes, Charles W. Gehrke, Robert W. Zumwalt, Sai Y. Chang, Duane B. Lakings, Douglass C. Tormey, David L. Ahmann, Charles G. Moertel

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

By means of a sensitive and specific method utilizing gas‐liquid chromatography, the excretion levels for three nucleosides, degradation minor base products of ribonucleic acid, primarily transfer ribonucleic acid, were determined in 24–hour urine specimens from over 200 patients with solid tumor malignancies. These nucleosides were N², N²‐dimethylguanosine, 1–methylinosine, and pseudouridine. When compared to normal control values, elevated levels of these compounds were found for patients in each of several tumor types studied. Increases in pseudouridine excretion suggest increased tumor transfer ribonucleic acid turnover; in addition, for the methylated nucleosides, higher than normal values may reflect enhanced transfer ribonucleic acid methylase activity of the neoplastic cells.

Original language	English (US)
Pages (from-to)	390-398
Number of pages	9
Journal	Cancer
Volume	36
Issue number	2
DOIs	https://doi.org/10.1002/1097-0142(197508)36:2<390::AID-CNCR2820360214>3.0.CO;2-C
State	Published - Aug 1975
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1002/1097-0142(197508)36:2<390::AID-CNCR2820360214>3.0.CO;2-C

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title = "The urinary excretion of nucleosides of ribonucleic acid by patients with advanced cancer",

abstract = "By means of a sensitive and specific method utilizing gas‐liquid chromatography, the excretion levels for three nucleosides, degradation minor base products of ribonucleic acid, primarily transfer ribonucleic acid, were determined in 24–hour urine specimens from over 200 patients with solid tumor malignancies. These nucleosides were N2, N2‐dimethylguanosine, 1–methylinosine, and pseudouridine. When compared to normal control values, elevated levels of these compounds were found for patients in each of several tumor types studied. Increases in pseudouridine excretion suggest increased tumor transfer ribonucleic acid turnover; in addition, for the methylated nucleosides, higher than normal values may reflect enhanced transfer ribonucleic acid methylase activity of the neoplastic cells.",

author = "Waalkes, {T. Phillip} and Gehrke, {Charles W.} and Zumwalt, {Robert W.} and Chang, {Sai Y.} and Lakings, {Duane B.} and Tormey, {Douglass C.} and Ahmann, {David L.} and Moertel, {Charles G.}",

year = "1975",

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doi = "10.1002/1097-0142(197508)36:2<390::AID-CNCR2820360214>3.0.CO;2-C",

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publisher = "John Wiley and Sons Inc.",

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T1 - The urinary excretion of nucleosides of ribonucleic acid by patients with advanced cancer

AU - Waalkes, T. Phillip

AU - Gehrke, Charles W.

AU - Zumwalt, Robert W.

AU - Chang, Sai Y.

AU - Lakings, Duane B.

AU - Tormey, Douglass C.

AU - Ahmann, David L.

AU - Moertel, Charles G.

PY - 1975/8

Y1 - 1975/8

N2 - By means of a sensitive and specific method utilizing gas‐liquid chromatography, the excretion levels for three nucleosides, degradation minor base products of ribonucleic acid, primarily transfer ribonucleic acid, were determined in 24–hour urine specimens from over 200 patients with solid tumor malignancies. These nucleosides were N2, N2‐dimethylguanosine, 1–methylinosine, and pseudouridine. When compared to normal control values, elevated levels of these compounds were found for patients in each of several tumor types studied. Increases in pseudouridine excretion suggest increased tumor transfer ribonucleic acid turnover; in addition, for the methylated nucleosides, higher than normal values may reflect enhanced transfer ribonucleic acid methylase activity of the neoplastic cells.

AB - By means of a sensitive and specific method utilizing gas‐liquid chromatography, the excretion levels for three nucleosides, degradation minor base products of ribonucleic acid, primarily transfer ribonucleic acid, were determined in 24–hour urine specimens from over 200 patients with solid tumor malignancies. These nucleosides were N2, N2‐dimethylguanosine, 1–methylinosine, and pseudouridine. When compared to normal control values, elevated levels of these compounds were found for patients in each of several tumor types studied. Increases in pseudouridine excretion suggest increased tumor transfer ribonucleic acid turnover; in addition, for the methylated nucleosides, higher than normal values may reflect enhanced transfer ribonucleic acid methylase activity of the neoplastic cells.

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The urinary excretion of nucleosides of ribonucleic acid by patients with advanced cancer

Abstract

ASJC Scopus subject areas

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