TY - JOUR
T1 - The UOK 257 cell line
T2 - a novel model for studies of the human Birt-Hogg-Dubé gene pathway
AU - Yang, Youfeng
AU - Padilla-Nash, Hesed M.
AU - Vira, Manish A.
AU - Abu-Asab, Mones S.
AU - Val, Daniel
AU - Worrell, Robert
AU - Tsokos, Maria
AU - Merino, Maria J.
AU - Pavlovich, Christian P.
AU - Ried, Thomas
AU - Linehan, W. Marston
AU - Vocke, Cathy D.
N1 - Funding Information:
This research was supported in part by the Intramural Research Program of the U.S. National Institutes of Health, National Cancer Institute, Center for Cancer Research. The authors are grateful to Linda Stapleton-Barenboim for preparation of the SKY probes and to Jianping Huang and Zhiyu Li for assisting in flow cytometric facilities.
PY - 2008/1/15
Y1 - 2008/1/15
N2 - The establishment, characterization, and tumorigenicity of a new epithelial cell line (UOK 257) derived from human renal carcinoma of an individual with Birt-Hogg-Dubé (BHD) syndrome are reported. Unlike other established renal tumor cell lines from sporadic renal cell carcinoma, this is the first established renal tumor cell line of BHD, an inheritable neoplastic syndrome. The isolated tumor cells display loss of contact inhibition in vitro, and produce subcutaneous tumors in mouse xenografts. Histopathologic, ultrastructural, and cytogenetic characterizations of the established tumor cells are reported. Cytogenetic analysis using spectral karyotyping on UOK 257 cells revealed 17p loss and a near-triploid and aneuploid karyotype with multiple fluorescence in situ hybridization analysis using a locus-specific gene probe for MYC. The result demonstrates that the established tumor cells consist of two cell populations, one containing four and one containing five copies of the MYC oncogene.
AB - The establishment, characterization, and tumorigenicity of a new epithelial cell line (UOK 257) derived from human renal carcinoma of an individual with Birt-Hogg-Dubé (BHD) syndrome are reported. Unlike other established renal tumor cell lines from sporadic renal cell carcinoma, this is the first established renal tumor cell line of BHD, an inheritable neoplastic syndrome. The isolated tumor cells display loss of contact inhibition in vitro, and produce subcutaneous tumors in mouse xenografts. Histopathologic, ultrastructural, and cytogenetic characterizations of the established tumor cells are reported. Cytogenetic analysis using spectral karyotyping on UOK 257 cells revealed 17p loss and a near-triploid and aneuploid karyotype with multiple fluorescence in situ hybridization analysis using a locus-specific gene probe for MYC. The result demonstrates that the established tumor cells consist of two cell populations, one containing four and one containing five copies of the MYC oncogene.
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U2 - 10.1016/j.cancergencyto.2007.10.010
DO - 10.1016/j.cancergencyto.2007.10.010
M3 - Article
C2 - 18206534
AN - SCOPUS:38149094921
SN - 0165-4608
VL - 180
SP - 100
EP - 109
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 2
ER -