The ultrastructural localization of secretory acid phosphatase in giant cell tumor of bone

Edward F McCarthy, J. A. Serrano, H. L. Wasserkrug, H. D. Dorfman

Research output: Contribution to journalArticle

Abstract

Three cases of giant cell tumor of bone were studied with the light and electron microscopes to determine the histochemical and cytochemical distribution of acid phosphatase isoenzymes. Using β-glycerophosphate as a nonspecific substrate, acid phosphatase was found in the giant cells as well as the stromal cells. Ultrastructurally, using this substrate, the enzyme was found to be associated with lysosomes in the stromal cells and giant cells and also with the profuse system of tubules and vesicles in the cytoplasm of the giant cells. Using phosphorylcholine and D-ephedrinephosphate, which are substrates for a specific secretory isoenzyme of acid phosphatase, activity was found only in the vesicles of the giant cell cytoplasm. Lysosomes did not show activity with these substrates. Multinucleated cells of giant cell tumor contain a specific secretory type of acid phosphatase which is not present in the stromal cells. This suggests that the giant cells are attempting to form a secretory system similar to osteoclasts and that the vesicles in the cytoplasm which contain this acid phosphates are the morphologic expression of an abortive secretory system.

Original languageEnglish (US)
Pages (from-to)295-302
Number of pages8
JournalClinical Orthopaedics and Related Research
VolumeNO 141
StatePublished - 1979

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Giant Cell Tumor of Bone
Giant Cells
Acid Phosphatase
Stromal Cells
Cytoplasm
Lysosomes
Isoenzymes
Glycerophosphates
Giant Cell Tumors
Phosphorylcholine
Osteoclasts
Phosphates
Electrons
Light
Acids
Enzymes

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

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The ultrastructural localization of secretory acid phosphatase in giant cell tumor of bone. / McCarthy, Edward F; Serrano, J. A.; Wasserkrug, H. L.; Dorfman, H. D.

In: Clinical Orthopaedics and Related Research, Vol. NO 141, 1979, p. 295-302.

Research output: Contribution to journalArticle

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