The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing

Susan Kass, Kazimierz Tyc, Joan A. Steitz, Barbara Sollner-Webb

Research output: Contribution to journalArticlepeer-review

Abstract

The first cleavage in mammalian pre-rRNA maturation occurs near the 5′ end within the 5′ external transcribed spacer. Using mouse cell extracts, we show that this processing is abolished by micrococcal nuclease pretreatment. Autoantibodies that recognize the U3, U8, and U13 snRNPs (anti-fibrillarin) deplete processing activity from the extract and selectively immunoprecipitate both rRNA substrates and processing products from the reaction. Specific involvement of the U3 snRNP is demonstrated by native gel electrophoresis of the processing reaction followed by Northern blotting and by oligonucleotide-directed RNA-ase H abolition of processing activity. Our identification of U3 function is discussed with respect to the molecular basis of pre-rRNA recognition by the U3 snRNP, possible roles of U3 and other nucleolar snRNPs in rRNA processing, and the morphological organization of the nucleolus and the ribosomal transcription complex.

Original languageEnglish (US)
Pages (from-to)897-908
Number of pages12
JournalCell
Volume60
Issue number6
DOIs
StatePublished - Mar 23 1990

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing'. Together they form a unique fingerprint.

Cite this