The tyrosine phosphatase, SHP-1, is involved in bronchial mucin production during oxidative stress

Min Kyoung Jang, Sae Hoon Kim, Ki Young Lee, Tae Bum Kim, Keun Ae Moon, Chan Sun Park, Yun Jeong Bae, Zhou Zhu, Hee Bom Moon, You Sook Cho

Research output: Contribution to journalArticle

Abstract

Mucus hypersecretion is a clinically important manifestation of chronic inflammatory airway diseases, such as asthma and Chronic obstructive pulmonary disease (COPD). Mucin production in airway epithelia is increased under conditions of oxidative stress. Src homology 2 domain-containing protein tyrosine phosphatase (SHP)-1 suppression is related to the development of airway inflammation and increased ROS levels. In this study, we investigated the role of SHP-1 in mucin secretion triggered by oxidative stress. Human lung mucoepidermoid H292 carcinoma cells were transfected with specific siRNA to eliminate SHP-1 gene expression. Cultured cells were treated with hydrogen peroxide (H2O2), and Mucin 5AC(MUC5AC) gene expression and mucin production were determined. Activation of p38 mitogen activated protein kinase (MAPK) in association with MUC5AC production was evaluated. N-acetylcysteine (NAC) was employed to determine whether antioxidants could block MUC5AC production. To establish the precise role of p38, mucin expression was observed after pre-treatment of SHP-1-depleted H292 cells with the p38 chemical blocker. We investigated the in vivo effects of oxidative stress on airway mucus production in SHP-1-deficient heterozygous (mev/+) mice. MUC5AC expression was enhanced in SHP-1 knockdown H292 cells exposed to H2O2, compared to that in control cells. The ratio between phosphorylated and total p38 was significantly increased in SHP-1-deficient cells under oxidative stress. Pre-treatment with NAC suppressed both MUC5AC production and p38 activation. Blockage of p38 MAPK led to suppression of MUC5AC mRNA expression. Notably, mucin production was enhanced in the airway epithelia of mev/+ mice exposed to oxidative stress. Our results clearly indicate that SHP-1 plays an important role in airway mucin production through regulating oxidative stress.

Original languageEnglish (US)
Pages (from-to)137-143
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume393
Issue number1
DOIs
StatePublished - Feb 26 2010

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Mucin 5AC
Non-Receptor Type 6 Protein Tyrosine Phosphatase
Oxidative stress
Mucins
Oxidative Stress
Acetylcysteine
p38 Mitogen-Activated Protein Kinases
Mucus
SH2 Domain-Containing Protein Tyrosine Phosphatases
Epithelium
Gene expression
Mucoepidermoid Carcinoma
Gene Expression
Mucin-1
Protein Tyrosine Phosphatases
Chemical activation
Cells
Pulmonary diseases
Chronic Obstructive Pulmonary Disease
Hydrogen Peroxide

Keywords

  • Asthma
  • Mucin
  • Oxidative stress
  • SHP-1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

The tyrosine phosphatase, SHP-1, is involved in bronchial mucin production during oxidative stress. / Jang, Min Kyoung; Kim, Sae Hoon; Lee, Ki Young; Kim, Tae Bum; Moon, Keun Ae; Park, Chan Sun; Bae, Yun Jeong; Zhu, Zhou; Moon, Hee Bom; Cho, You Sook.

In: Biochemical and Biophysical Research Communications, Vol. 393, No. 1, 26.02.2010, p. 137-143.

Research output: Contribution to journalArticle

Jang, MK, Kim, SH, Lee, KY, Kim, TB, Moon, KA, Park, CS, Bae, YJ, Zhu, Z, Moon, HB & Cho, YS 2010, 'The tyrosine phosphatase, SHP-1, is involved in bronchial mucin production during oxidative stress', Biochemical and Biophysical Research Communications, vol. 393, no. 1, pp. 137-143. https://doi.org/10.1016/j.bbrc.2010.01.102
Jang, Min Kyoung ; Kim, Sae Hoon ; Lee, Ki Young ; Kim, Tae Bum ; Moon, Keun Ae ; Park, Chan Sun ; Bae, Yun Jeong ; Zhu, Zhou ; Moon, Hee Bom ; Cho, You Sook. / The tyrosine phosphatase, SHP-1, is involved in bronchial mucin production during oxidative stress. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 393, No. 1. pp. 137-143.
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AB - Mucus hypersecretion is a clinically important manifestation of chronic inflammatory airway diseases, such as asthma and Chronic obstructive pulmonary disease (COPD). Mucin production in airway epithelia is increased under conditions of oxidative stress. Src homology 2 domain-containing protein tyrosine phosphatase (SHP)-1 suppression is related to the development of airway inflammation and increased ROS levels. In this study, we investigated the role of SHP-1 in mucin secretion triggered by oxidative stress. Human lung mucoepidermoid H292 carcinoma cells were transfected with specific siRNA to eliminate SHP-1 gene expression. Cultured cells were treated with hydrogen peroxide (H2O2), and Mucin 5AC(MUC5AC) gene expression and mucin production were determined. Activation of p38 mitogen activated protein kinase (MAPK) in association with MUC5AC production was evaluated. N-acetylcysteine (NAC) was employed to determine whether antioxidants could block MUC5AC production. To establish the precise role of p38, mucin expression was observed after pre-treatment of SHP-1-depleted H292 cells with the p38 chemical blocker. We investigated the in vivo effects of oxidative stress on airway mucus production in SHP-1-deficient heterozygous (mev/+) mice. MUC5AC expression was enhanced in SHP-1 knockdown H292 cells exposed to H2O2, compared to that in control cells. The ratio between phosphorylated and total p38 was significantly increased in SHP-1-deficient cells under oxidative stress. Pre-treatment with NAC suppressed both MUC5AC production and p38 activation. Blockage of p38 MAPK led to suppression of MUC5AC mRNA expression. Notably, mucin production was enhanced in the airway epithelia of mev/+ mice exposed to oxidative stress. Our results clearly indicate that SHP-1 plays an important role in airway mucin production through regulating oxidative stress.

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