The type III secretion system is involved in the invasion and intracellular survival of Escherichia coli K1 in human brain microvascular endothelial cells

Yufeng Yao, Yi Xie, Donna Perace, Yi Zhong, Jie Lu, Jing Tao, Xiaokui Guo, Kwang Sik Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Type III secretion systems (T3SSs) have been documented in many Gram-negative bacteria, including enterohemorrhagic Escherichia coli. We have previously shown the existence of a putative T3SS in meningitis-causing E. coli K1 strains, referred to as E. coli type III secretion 2 (ETT2). The sequence of ETT2 in meningitis-causing E. coli K1 strain EC10 (O7:K1) revealed that ETT2 comprises the epr, epa and eiv genes, but bears mutations, deletions and insertions. We constructed the EC10 mutants deleted of ETT2 or eivA gene, and their contributions to bacterial pathogenesis were evaluated in human brain microvascular endothelial cells (HBMECs). The deletion mutant of ETT2 exhibited defects in invasion and intracellular survival compared with the parental E. coli K1 strain EC10. The mutant deleted of eivA within ETT2 was also significantly defective in invasion and intracellular survival in HBMECs, and the defects of the eiv mutant were restored to the levels of the parent strain EC10 by transcomplementation. These findings suggest that ETT2 plays a role in the pathogenesis of E. coli K1 infection, including meningitis.

Original languageEnglish (US)
Pages (from-to)18-24
Number of pages7
JournalFEMS microbiology letters
Volume300
Issue number1
DOIs
StatePublished - Nov 2009

Keywords

  • Blood-brain barrier
  • Escherichia coli K1
  • Meningitis
  • Type III secretion system

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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