TY - JOUR
T1 - The transmembrane protein off-track associates with plexins and functions downstream of semaphorin signaling during axon guidance
AU - Winberg, Margaret L.
AU - Tamagnone, Luca
AU - Bai, Jianwu
AU - Comoglio, Paolo M.
AU - Montell, Denise
AU - Goodman, Corey S.
N1 - Funding Information:
We thank Paola Angelini for enthusiastic help in the Plexin-OTK association experiments. Supported by National Institutes of Health grant HD21294 (C.S.G.), the Armenise-Harvard Foundation for Advanced Scientific Research (P.M.C.), a grant from the March of Dimes (D.M.), and by Telethon-Italy grant E-1129 (to L.T.). C.S.G. was an Investigator and M.L.W. was a Postdoctoral Fellow with the Howard Hughes Medical Institute.
PY - 2001
Y1 - 2001
N2 - The Plexin family of transmembrane proteins appears to function as repulsive receptors for most if not all Semaphorins. Here, we use genetic and biochemical analysis in Drosophila to show that the transmembrane protein Off-track (OTK) associates with Plexin A, the receptor for Sema 1a, and that OTK is a component of the repulsive signaling response to Semaphorin ligands. In vitro, OTK associates with Plexins. In vivo, mutations in the otk gene lead to phenotypes resembling those of loss-of-function mutations of either Sema1a or PlexA. The otk gene displays strong genetic interactions with Sema1a and PlexA, suggesting that OTK and Plexin A function downstream of Sema 1a.
AB - The Plexin family of transmembrane proteins appears to function as repulsive receptors for most if not all Semaphorins. Here, we use genetic and biochemical analysis in Drosophila to show that the transmembrane protein Off-track (OTK) associates with Plexin A, the receptor for Sema 1a, and that OTK is a component of the repulsive signaling response to Semaphorin ligands. In vitro, OTK associates with Plexins. In vivo, mutations in the otk gene lead to phenotypes resembling those of loss-of-function mutations of either Sema1a or PlexA. The otk gene displays strong genetic interactions with Sema1a and PlexA, suggesting that OTK and Plexin A function downstream of Sema 1a.
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U2 - 10.1016/S0896-6273(01)00446-9
DO - 10.1016/S0896-6273(01)00446-9
M3 - Article
C2 - 11604138
AN - SCOPUS:0034782822
SN - 0896-6273
VL - 32
SP - 53
EP - 62
JO - Neuron
JF - Neuron
IS - 1
ER -