The transcription factor c-jun is activated in retinal ganglion cells in experimental rat glaucoma

Hana Levkovitch-Verbin, Harry A Quigley, Keith R G Martin, Noga Harizman, Danielle F. Valenta, Mary Ellen Pease, Shlomo Melamed

Research output: Contribution to journalArticle

Abstract

This study investigates the role of the MAP kinase pathway including c-jun, ATF-2 and JNK in glaucomatous eyes of rats and in optic nerve transection. Glaucoma was induced in one eye of 51 adult Wistar rats by laser treatment to the trabecular meshwork. Eighteen further rats underwent unilateral optic nerve transection. We studied the transcription factor c-jun, its activated form, phospho-c-jun, the transcription factor p-ATF-2, and the enzyme JNK by immunohistochemistry. The activation of p-c-jun was also investigated using western blot analysis. Treated and control eyes were compared in a masked way at multiple time points after injury. We found a statistically significant increase in immunolabelling for c-jun and phospho-c-jun in retinal ganglion cells (RGCs) from 1 day to 4 weeks after intraocular pressure (IOP) elevation. At 1 and 2 days after the laser treatment, a mean of 2.9±3.3 RGCs mm -1 were positive for c-jun (n=12, p=0.005, t-test), increasing to a mean of 13.4±7.5 cells mm-1 at 1 week (n=18, p=0.00005), and decreasing to 2.3±2.0 cells mm-1 at 2 weeks (n=5, p=0.04) and 0.1±0.1 cells mm-1 at 2 months. Few of the 47 control eyes had any labelling for c-jun or phospho-c-jun, while between 80 and 100% of elevated IOP eyes showed positivity during the first 2 weeks of experimental glaucoma. After optic nerve transection, c-jun and phospho-c-jun were also significantly activated at 1, 2 and 9 days (p

Original languageEnglish (US)
Pages (from-to)663-670
Number of pages8
JournalExperimental Eye Research
Volume80
Issue number5
DOIs
StatePublished - May 2005

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Retinal Ganglion Cells
Glaucoma
Optic Nerve Injuries
Transcription Factors
Intraocular Pressure
Lasers
Activating Transcription Factors
Trabecular Meshwork
Wistar Rats
Phosphotransferases
Western Blotting
Immunohistochemistry
Wounds and Injuries
Enzymes
Therapeutics

Keywords

  • c-jun
  • Glaucoma
  • JNK
  • Optic nerve transection
  • Retinal ganglion cells

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

The transcription factor c-jun is activated in retinal ganglion cells in experimental rat glaucoma. / Levkovitch-Verbin, Hana; Quigley, Harry A; Martin, Keith R G; Harizman, Noga; Valenta, Danielle F.; Pease, Mary Ellen; Melamed, Shlomo.

In: Experimental Eye Research, Vol. 80, No. 5, 05.2005, p. 663-670.

Research output: Contribution to journalArticle

Levkovitch-Verbin, Hana ; Quigley, Harry A ; Martin, Keith R G ; Harizman, Noga ; Valenta, Danielle F. ; Pease, Mary Ellen ; Melamed, Shlomo. / The transcription factor c-jun is activated in retinal ganglion cells in experimental rat glaucoma. In: Experimental Eye Research. 2005 ; Vol. 80, No. 5. pp. 663-670.
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