TY - JOUR
T1 - The Transactivating Domain of the c-Jun Proto-Oncoprotein Is Required for Cotransformation of Rat Embryo Cells
AU - Alani, Rhoda
AU - Brown, Powel
AU - Binétruy, Bernard
AU - Dosaka, Hirotoshi
AU - Rosenberg, Richard K.
AU - Angel, Peter
AU - Karin, Michael
AU - Birrer, Michael J.
PY - 1991/12
Y1 - 1991/12
N2 - The nuclear phosphoprotein c-Jun, encoded by the proto-oncogene c-jun, is a major component of the AP-1 complex. A potent transcriptional regulator, c-jun is also able to transform normal rat embryo cells in cooperation with an activated c-Ha-ras gene. By deletion analysis, we identified the regions of c-Jun encoding transformation and transactivation functions. Our studies indicate that there is a direct correlation between the ability of the c-Jun protein to activate transcription and cotransform rat embryo cells. The regions involved in these functions include the conserved leucine zipper/DNA binding domain and an effector domain near its N terminus. This N-terminal region spans amino acids 61 to 146 of the c-Jun protein and is highly conserved among all Jun family members. These results support the hypothesis that c-Jun transforms cells by stimulating the expression of transformation-mediating genes.
AB - The nuclear phosphoprotein c-Jun, encoded by the proto-oncogene c-jun, is a major component of the AP-1 complex. A potent transcriptional regulator, c-jun is also able to transform normal rat embryo cells in cooperation with an activated c-Ha-ras gene. By deletion analysis, we identified the regions of c-Jun encoding transformation and transactivation functions. Our studies indicate that there is a direct correlation between the ability of the c-Jun protein to activate transcription and cotransform rat embryo cells. The regions involved in these functions include the conserved leucine zipper/DNA binding domain and an effector domain near its N terminus. This N-terminal region spans amino acids 61 to 146 of the c-Jun protein and is highly conserved among all Jun family members. These results support the hypothesis that c-Jun transforms cells by stimulating the expression of transformation-mediating genes.
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M3 - Article
C2 - 1944289
AN - SCOPUS:0026060618
SN - 0270-7306
VL - 11
SP - 6286
EP - 6295
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 12
ER -