The Transactivating Domain of the c-Jun Proto-Oncoprotein Is Required for Cotransformation of Rat Embryo Cells

Rhoda Alani, Powel Brown, Bernard Binétruy, Hirotoshi Dosaka, Richard K. Rosenberg, Peter Angel, Michael Karin, Michael J. Birrer

Research output: Contribution to journalArticle

Abstract

The nuclear phosphoprotein c-Jun, encoded by the proto-oncogene c-jun, is a major component of the AP-1 complex. A potent transcriptional regulator, c-jun is also able to transform normal rat embryo cells in cooperation with an activated c-Ha-ras gene. By deletion analysis, we identified the regions of c-Jun encoding transformation and transactivation functions. Our studies indicate that there is a direct correlation between the ability of the c-Jun protein to activate transcription and cotransform rat embryo cells. The regions involved in these functions include the conserved leucine zipper/DNA binding domain and an effector domain near its N terminus. This N-terminal region spans amino acids 61 to 146 of the c-Jun protein and is highly conserved among all Jun family members. These results support the hypothesis that c-Jun transforms cells by stimulating the expression of transformation-mediating genes.

Original languageEnglish (US)
Pages (from-to)6286-6295
Number of pages10
JournalMolecular and Cellular Biology
Volume11
Issue number12
StatePublished - Dec 1991
Externally publishedYes

Fingerprint

Oncogene Proteins
Proto-Oncogene Proteins c-jun
Embryonic Structures
jun Genes
Leucine Zippers
Aptitude
ras Genes
Phosphoproteins
Transcription Factor AP-1
Transcriptional Activation
Amino Acids
DNA
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Alani, R., Brown, P., Binétruy, B., Dosaka, H., Rosenberg, R. K., Angel, P., ... Birrer, M. J. (1991). The Transactivating Domain of the c-Jun Proto-Oncoprotein Is Required for Cotransformation of Rat Embryo Cells. Molecular and Cellular Biology, 11(12), 6286-6295.

The Transactivating Domain of the c-Jun Proto-Oncoprotein Is Required for Cotransformation of Rat Embryo Cells. / Alani, Rhoda; Brown, Powel; Binétruy, Bernard; Dosaka, Hirotoshi; Rosenberg, Richard K.; Angel, Peter; Karin, Michael; Birrer, Michael J.

In: Molecular and Cellular Biology, Vol. 11, No. 12, 12.1991, p. 6286-6295.

Research output: Contribution to journalArticle

Alani, R, Brown, P, Binétruy, B, Dosaka, H, Rosenberg, RK, Angel, P, Karin, M & Birrer, MJ 1991, 'The Transactivating Domain of the c-Jun Proto-Oncoprotein Is Required for Cotransformation of Rat Embryo Cells', Molecular and Cellular Biology, vol. 11, no. 12, pp. 6286-6295.
Alani R, Brown P, Binétruy B, Dosaka H, Rosenberg RK, Angel P et al. The Transactivating Domain of the c-Jun Proto-Oncoprotein Is Required for Cotransformation of Rat Embryo Cells. Molecular and Cellular Biology. 1991 Dec;11(12):6286-6295.
Alani, Rhoda ; Brown, Powel ; Binétruy, Bernard ; Dosaka, Hirotoshi ; Rosenberg, Richard K. ; Angel, Peter ; Karin, Michael ; Birrer, Michael J. / The Transactivating Domain of the c-Jun Proto-Oncoprotein Is Required for Cotransformation of Rat Embryo Cells. In: Molecular and Cellular Biology. 1991 ; Vol. 11, No. 12. pp. 6286-6295.
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