The topology of the kinetoplast DNA network

Junghuei Chen, Carol A. Rauch, James H. White, Paul T. Englund, Nicholas R. Cozzarelli

Research output: Contribution to journalArticlepeer-review

Abstract

Kinetoplast DNA (kDNA) of trypanosomatid parasites is a network of ∼5000 catenated DNA minicircles and ∼25 maxicircles. We developed the following strategy to deduce the topological linkage of the minicircles of the Crithidia fasciculata network. First, we used graph theory to provide precise models of possible network structures. Second, on the basis of these models, we predicted the frequencies of minicircle oligomers expected from random network breakage. Third, we determined the fragmentation pattern of kDNA networks as a function of the extent of digestion. Fourth, by comparison of the results with the predictions, we identified the model that best represents the network. We conclude that each minicircle is linked on average to three other minicircles. A honeycomb arrangement probably results, with each minicircle typically at the vertex of a hexagonal cell. This topology has implications for the assembly, structure, and function of kDNA networks.

Original languageEnglish (US)
Pages (from-to)61-69
Number of pages9
JournalCell
Volume80
Issue number1
DOIs
StatePublished - Jan 13 1995

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint

Dive into the research topics of 'The topology of the kinetoplast DNA network'. Together they form a unique fingerprint.

Cite this