@article{8d8c00f825e843ce9d4104898901a557,
title = "The Toll immune signaling pathway control conserved anti-dengue defenses across diverse Ae. aegypti strains and against multiple dengue virus serotypes",
abstract = "Dengue virus has become one of the most important arboviral pathogens affecting the world today. The virus is transmitted among humans by the mosquitoes Aedes aegypti and Ae. albopictus. Like other vector-borne pathogens, this virus encounters innate immune defenses within the mosquito vector that limit infection. We have previously demonstrated the involvement of the Toll pathway in the anti-dengue defense at 7 days after infection. In the present study, we have investigated the activity of this immune signaling pathway against different dengue virus serotypes at the early stages of infection in laboratory and field-derived mosquito strains. Our studies corroborate the importance of the Toll pathway in the anti-dengue defense repertoire at 3 days after an infectious blood meal, when new virions are released from the midgut for dissemination and infection of other mosquito tissues. These immune defenses are furthermore conserved among different Ae. aegypti strains and can act against a broad range of dengue virus serotypes.",
keywords = "Arbovirus, Dengue, Innate immunity, Toll pathway",
author = "Ramirez, {Jose L.} and George Dimopoulos",
note = "Funding Information: We are grateful to Janece M. Lovchik, Bhavin Thumar and Anna P. Durbin for providing the techniques support and the materials (DENV-2 and DENV-4 strains and C6/36 cell line), and for sharing the equipment (CO2 incubator and fluorescence microscope) for this study. We are also thankful to the Arbovirus Diseases Branch at the CDC for providing us with the anti-dengue antibodies (Mouse Hyperimmune Ascetic Fluid). We thank the microarray core facility and the insectary personnel at the Johns Hopkins Malaria Research Institute for assistance with the microarray assays and mosquito rearing. We also thank Dr. Deborah McClellan for editorial assistance. We thank Dr. Jorge Motta, Anayansi Valderrama and the Gorgas Institute for providing field-derived Ae. aegypti strains. This work has been supported by the National Institutes of Health/National Institute for Allergy and Infectious Disease 1R01AI061576-01A1 , RO1AI059492 , RO1AI078997 , the Johns Hopkins School of Public Health and the Johns Hopkins Malaria Research Institute . JLR was supported by an individual F31 NRSA training grant from NIH/NIAAD (1F31AI080161-01A1) and by the American Society for Microbiology Robert D. Watkins Graduate Research Fellowship. ",
year = "2010",
month = jun,
doi = "10.1016/j.dci.2010.01.006",
language = "English (US)",
volume = "34",
pages = "625--629",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier Limited",
number = "6",
}