The three-dimensional structure of an arachidonic acid 15-lipoxygenase

Jeffrey C. Boyington, Betty J. Gaffney, Mario L Amzel

Research output: Contribution to journalArticle

Abstract

In mammals, the hydroperoxidation of arachidonic acid by lipoxygenases leads to the formation of leukotrienes and lipoxins, compounds that mediate inflammatory responses. Lipoxygenases are dioxygenases that contain a nonheme iron and are present in many animal cells. Soybean lipoxygenase-1 is a single-chain, 839-residue protein closely related to mammalian lipoxygenases. The structure of soybean lipoxygenase-1 solved to 2.6 angstrom resolution shows that the enzyme has two domains: a 146-residue β barrel and a 693-residue helical bundle. The iron atom is in the center of the larger domain and is coordinated by three histidines and the COO- of the carboxyl terminus. The coordination geometry is nonregular and appears to be a distorted octahedron in which two adjacent positions are not occupied by ligands. Two cavities, in the shapes of a bent cylinder and a frustum, connect the unoccupied positions to the surface of the enzyme. The iron, with two adjacent and unoccupied positions, is poised to interact with the 1,4-diene system of the substrate and with molecular oxygen during catalysis.

Original languageEnglish (US)
Pages (from-to)1482-1486
Number of pages5
JournalScience
Volume260
Issue number5113
StatePublished - 1993

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Arachidonate 15-Lipoxygenase
Lipoxygenases
Iron
Lipoxins
Arachidonate Lipoxygenases
Dioxygenases
Leukotrienes
Enzymes
Catalysis
Histidine
Mammals
Oxygen
Ligands
Proteins
lipoxygenase L-1

ASJC Scopus subject areas

  • General

Cite this

Boyington, J. C., Gaffney, B. J., & Amzel, M. L. (1993). The three-dimensional structure of an arachidonic acid 15-lipoxygenase. Science, 260(5113), 1482-1486.

The three-dimensional structure of an arachidonic acid 15-lipoxygenase. / Boyington, Jeffrey C.; Gaffney, Betty J.; Amzel, Mario L.

In: Science, Vol. 260, No. 5113, 1993, p. 1482-1486.

Research output: Contribution to journalArticle

Boyington, JC, Gaffney, BJ & Amzel, ML 1993, 'The three-dimensional structure of an arachidonic acid 15-lipoxygenase', Science, vol. 260, no. 5113, pp. 1482-1486.
Boyington, Jeffrey C. ; Gaffney, Betty J. ; Amzel, Mario L. / The three-dimensional structure of an arachidonic acid 15-lipoxygenase. In: Science. 1993 ; Vol. 260, No. 5113. pp. 1482-1486.
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