The systemic administration of purified interleukin 2 enhances the ability of sensitized murine lymphocytes to cure a disseminated syngeneic lymphoma

J. H. Donohue, M. Rosenstein, A. E. Chang, M. T. Lotze, R. J. Robb, S. A. Rosenberg

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Abstract

Significant serum titers of interleukin 2 (IL 2) can be maintained in mice for 12 h after i.p. injection in a 15% gelatin solution. We have tested the ability of IL 2 administered systemically in this fashion to enhance the therapeutic effect of adoptively transferred specifically sensitized lymphoid cells that were expanded in IL 2. Mice with established local and disseminated FBL-3 lymphoma, induced by intrafootpad injection of 107 cells after 500 rad total body irradiation, were treated with a combination of i.v. injected murine spenocytes and either murine supernatants containing IL 2 or pure human IL 2 in gelatin. Splenocytes from immune mice were resensitized to irradiated tumor in vitro and were expanded for 7 days in lectin-free IL 2 supernatants. Treatment with these murine splenocytes administered with murine IL 2 supernatants prolonged mean survival to 33.6 days compared with mean survival times of 16.9 days (p <0.001) and 23.4 days (p = 0.007) for mice treated with IL 2 alone or splenocytes alone. Human IL 2, purified to homogeneity from the Jurkat cell line, was also capable of improving the therapeutic efficacy of transferred cells in mice. Mean survival was significantly prolonged to 32.1 days when cells and purified human IL 2 were administered, whereas mean survival times of 18.1 days (p +

Original languageEnglish (US)
Pages (from-to)2123-2128
Number of pages6
JournalJournal of Immunology
Volume132
Issue number4
Publication statusPublished - 1984
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

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Donohue, J. H., Rosenstein, M., Chang, A. E., Lotze, M. T., Robb, R. J., & Rosenberg, S. A. (1984). The systemic administration of purified interleukin 2 enhances the ability of sensitized murine lymphocytes to cure a disseminated syngeneic lymphoma. Journal of Immunology, 132(4), 2123-2128.