The synthesis of Gly-L-His-L-Lys (GHL), Gly-L-His-L-Lys: Copper (GHL- Cu) and their effect on DNA synthesis in cells

J. H. Chen; W. G. Wang; F. Q. Zhou; M. Shi

The synthesis of Gly-L-His-L-Lys (GHL), Gly-L-His-L-Lys: Copper (GHL- Cu) and their effect on DNA synthesis in cells

J. H. Chen, W. G. Wang, F. Q. Zhou, M. Shi

Research output: Contribution to journal › Article › peer-review

Abstract

AIM: To investigate the biological functions of GHL and GHL-Cu. METHODS: The GHL was synthesized by liquid peptide synthesis and cell DNA synthesis was measured by ³H-TdR incorporation. RESULTS: The effect of GHL and GHL-Cu on DNA synthesis in four different kinds of cells were tested. In low-serum culture condition, the GHL and GHL-Cu have promoting DNA synthesis in human epidermoid carcinoma (A431), human hepatoma cells (7721), human umbilical vein endothelial cells (HUVEC) and human fibroblast (HFB). The maximal effects are achieved at the concentration of 5 x 10^-8 ~ 5 x 10^-7 mol · L^-1 of GHL and GHL-Cu. CONCLUSION: The results provided useful data for explaining the mechanism of GHL-Cu to accelerate the healing of wounds.

Original language	English (US)
Pages (from-to)	455-457
Number of pages	3
Journal	Chinese Pharmacological Bulletin
Volume	13
Issue number	5
State	Published - Jan 1 1997
Externally published	Yes

Keywords

CHL-Cu
Cell culture
GHL
H-TdR incorporation

ASJC Scopus subject areas

Pharmacology

Cite this

@article{b8f107dc78cb4d83bac76e7df06575a0,

title = "The synthesis of Gly-L-His-L-Lys (GHL), Gly-L-His-L-Lys: Copper (GHL- Cu) and their effect on DNA synthesis in cells",

abstract = "AIM: To investigate the biological functions of GHL and GHL-Cu. METHODS: The GHL was synthesized by liquid peptide synthesis and cell DNA synthesis was measured by 3H-TdR incorporation. RESULTS: The effect of GHL and GHL-Cu on DNA synthesis in four different kinds of cells were tested. In low-serum culture condition, the GHL and GHL-Cu have promoting DNA synthesis in human epidermoid carcinoma (A431), human hepatoma cells (7721), human umbilical vein endothelial cells (HUVEC) and human fibroblast (HFB). The maximal effects are achieved at the concentration of 5 x 10-8 ~ 5 x 10-7 mol · L-1 of GHL and GHL-Cu. CONCLUSION: The results provided useful data for explaining the mechanism of GHL-Cu to accelerate the healing of wounds.",

keywords = "CHL-Cu, Cell culture, GHL, H-TdR incorporation",

author = "Chen, {J. H.} and Wang, {W. G.} and Zhou, {F. Q.} and M. Shi",

year = "1997",

month = jan,

day = "1",

language = "English (US)",

volume = "13",

pages = "455--457",

journal = "Chinese Pharmacological Bulletin",

issn = "1001-1978",

publisher = "Institute of Clinical Pharmacology",

number = "5",

}

TY - JOUR

T1 - The synthesis of Gly-L-His-L-Lys (GHL), Gly-L-His-L-Lys

T2 - Copper (GHL- Cu) and their effect on DNA synthesis in cells

AU - Chen, J. H.

AU - Wang, W. G.

AU - Zhou, F. Q.

AU - Shi, M.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - AIM: To investigate the biological functions of GHL and GHL-Cu. METHODS: The GHL was synthesized by liquid peptide synthesis and cell DNA synthesis was measured by 3H-TdR incorporation. RESULTS: The effect of GHL and GHL-Cu on DNA synthesis in four different kinds of cells were tested. In low-serum culture condition, the GHL and GHL-Cu have promoting DNA synthesis in human epidermoid carcinoma (A431), human hepatoma cells (7721), human umbilical vein endothelial cells (HUVEC) and human fibroblast (HFB). The maximal effects are achieved at the concentration of 5 x 10-8 ~ 5 x 10-7 mol · L-1 of GHL and GHL-Cu. CONCLUSION: The results provided useful data for explaining the mechanism of GHL-Cu to accelerate the healing of wounds.

AB - AIM: To investigate the biological functions of GHL and GHL-Cu. METHODS: The GHL was synthesized by liquid peptide synthesis and cell DNA synthesis was measured by 3H-TdR incorporation. RESULTS: The effect of GHL and GHL-Cu on DNA synthesis in four different kinds of cells were tested. In low-serum culture condition, the GHL and GHL-Cu have promoting DNA synthesis in human epidermoid carcinoma (A431), human hepatoma cells (7721), human umbilical vein endothelial cells (HUVEC) and human fibroblast (HFB). The maximal effects are achieved at the concentration of 5 x 10-8 ~ 5 x 10-7 mol · L-1 of GHL and GHL-Cu. CONCLUSION: The results provided useful data for explaining the mechanism of GHL-Cu to accelerate the healing of wounds.

KW - CHL-Cu

KW - Cell culture

KW - GHL

KW - H-TdR incorporation

UR - http://www.scopus.com/inward/record.url?scp=0031438046&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031438046&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0031438046

SN - 1001-1978

VL - 13

SP - 455

EP - 457

JO - Chinese Pharmacological Bulletin

JF - Chinese Pharmacological Bulletin

IS - 5

ER -

The synthesis of Gly-L-His-L-Lys (GHL), Gly-L-His-L-Lys: Copper (GHL- Cu) and their effect on DNA synthesis in cells

Abstract

Keywords

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this