AIM: To investigate the biological functions of GHL and GHL-Cu. METHODS: The GHL was synthesized by liquid peptide synthesis and cell DNA synthesis was measured by 3H-TdR incorporation. RESULTS: The effect of GHL and GHL-Cu on DNA synthesis in four different kinds of cells were tested. In low-serum culture condition, the GHL and GHL-Cu have promoting DNA synthesis in human epidermoid carcinoma (A431), human hepatoma cells (7721), human umbilical vein endothelial cells (HUVEC) and human fibroblast (HFB). The maximal effects are achieved at the concentration of 5 x 10-8 ~ 5 x 10-7 mol · L-1 of GHL and GHL-Cu. CONCLUSION: The results provided useful data for explaining the mechanism of GHL-Cu to accelerate the healing of wounds.
|Original language||English (US)|
|Number of pages||3|
|Journal||Chinese Pharmacological Bulletin|
|State||Published - Jan 1 1997|
- Cell culture
- H-TdR incorporation
ASJC Scopus subject areas