The synthesis of Gly-L-His-L-Lys (GHL), Gly-L-His-L-Lys: Copper (GHL- Cu) and their effect on DNA synthesis in cells

J. H. Chen, W. G. Wang, F. Q. Zhou, M. Shi

Research output: Contribution to journalArticle

Abstract

AIM: To investigate the biological functions of GHL and GHL-Cu. METHODS: The GHL was synthesized by liquid peptide synthesis and cell DNA synthesis was measured by 3H-TdR incorporation. RESULTS: The effect of GHL and GHL-Cu on DNA synthesis in four different kinds of cells were tested. In low-serum culture condition, the GHL and GHL-Cu have promoting DNA synthesis in human epidermoid carcinoma (A431), human hepatoma cells (7721), human umbilical vein endothelial cells (HUVEC) and human fibroblast (HFB). The maximal effects are achieved at the concentration of 5 x 10-8 ~ 5 x 10-7 mol · L-1 of GHL and GHL-Cu. CONCLUSION: The results provided useful data for explaining the mechanism of GHL-Cu to accelerate the healing of wounds.

Original languageEnglish (US)
Pages (from-to)455-457
Number of pages3
JournalChinese Pharmacological Bulletin
Volume13
Issue number5
StatePublished - Jan 1 1997
Externally publishedYes

Keywords

  • CHL-Cu
  • Cell culture
  • GHL
  • H-TdR incorporation

ASJC Scopus subject areas

  • Pharmacology

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