The synaptic SNARE complex is a parallel four-stranded helical bundle

M. A. Poirier, W. Xiao, J. C. Macosko, C. Chan, Y. K. Shin, M. K. Bennett

Research output: Contribution to journalArticle

Abstract

The heterotrimeric synaptic soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, consisting of the synaptic vesicle-associated membrane protein 2 (VAMP2) and presynaptic plasma membrane proteins SNAP-25 (synaptosome-associated protein of 25,000 M(r)) and syntaxin 1A, is a critical component of the exocytotic machinery. We have used spin labeling electron paramagnetic resonance spectroscopy to investigate the structural organization of this complex, particularly the two predicted helical domains contributed by SNAP-25. Our results indicate that the N- and C-terminal domains of SNAP-25 are parallel to each other and to the C- terminal domain of syntaxin 1A. Based on these findings, we propose a parallel four-stranded coiled coil model for the structure of the synaptic SNARE complex.

Original languageEnglish (US)
Pages (from-to)765-769
Number of pages5
JournalNature Structural Biology
Volume5
Issue number9
DOIs
StatePublished - 1998
Externally publishedYes

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SNARE Proteins
Syntaxin 1
Vesicle-Associated Membrane Protein 2
Synaptosomes
Synaptic Vesicles
Electron Spin Resonance Spectroscopy
Cell membranes
Labeling
Machinery
Paramagnetic resonance
Blood Proteins
Spectrum Analysis
Membrane Proteins
Cell Membrane
Spectroscopy
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Poirier, M. A., Xiao, W., Macosko, J. C., Chan, C., Shin, Y. K., & Bennett, M. K. (1998). The synaptic SNARE complex is a parallel four-stranded helical bundle. Nature Structural Biology, 5(9), 765-769. https://doi.org/10.1038/1799

The synaptic SNARE complex is a parallel four-stranded helical bundle. / Poirier, M. A.; Xiao, W.; Macosko, J. C.; Chan, C.; Shin, Y. K.; Bennett, M. K.

In: Nature Structural Biology, Vol. 5, No. 9, 1998, p. 765-769.

Research output: Contribution to journalArticle

Poirier, MA, Xiao, W, Macosko, JC, Chan, C, Shin, YK & Bennett, MK 1998, 'The synaptic SNARE complex is a parallel four-stranded helical bundle', Nature Structural Biology, vol. 5, no. 9, pp. 765-769. https://doi.org/10.1038/1799
Poirier MA, Xiao W, Macosko JC, Chan C, Shin YK, Bennett MK. The synaptic SNARE complex is a parallel four-stranded helical bundle. Nature Structural Biology. 1998;5(9):765-769. https://doi.org/10.1038/1799
Poirier, M. A. ; Xiao, W. ; Macosko, J. C. ; Chan, C. ; Shin, Y. K. ; Bennett, M. K. / The synaptic SNARE complex is a parallel four-stranded helical bundle. In: Nature Structural Biology. 1998 ; Vol. 5, No. 9. pp. 765-769.
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