The Symphony™ protocol for H&E staining of prostatic adenocarcinoma on needle biopsy: A multicentre analysis of 120 cases

Adeboye O. Osunkoya, Donna E. Hansel, Anil V. Parwani, Tehmina Z. Ali, Ecaterina F. Tamas, Vasundhara G. Untawale, Hillel Kahane, Jonathan I. Epstein

Research output: Contribution to journalArticle

Abstract

Aims: The diagnosis of prostate carcinoma is based on a constellation of architectural, nuclear, cytoplasmic, and ancillary features. The aim of this study was to determine if the Ventana Symphony H&E protocol can improve on the detection of prominent nucleoli in foci of prostate cancer. Methods: One hundred and twenty cases of Gleason score 3 + 3 = 6 cancers involving 10-30% of one core were retrieved from four academic institutions and two large laboratories (20 cases per institution). The routine H&E and Symphony™ H&E protocol stained slides from the same case were reviewed centrally in a blinded fashion at the Johns Hopkins Hospital. The mean percentages of prominent nucleoli in malignant and benign glands by visual estimation were recorded for each case. After unblinding, the routine H&E slides were compared in a pair-wise t-test with the corresponding Symphony™ stained slide. Results: Within all sites, the Symphony™ slides showed statistically significantly more prominent nucleoli in the malignant glands than the routine slides. Conclusions: The Symphony™ H&E staining protocol consistently highlights nucleoli in cancer to a greater extent than routine H&E stains and may increase the likelihood of making a diagnosis of limited adenocarcinoma of the prostate in challenging cases.

Original languageEnglish (US)
Pages (from-to)450-456
Number of pages7
JournalPathology
Volume40
Issue number5
DOIs
StatePublished - Aug 2008

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Keywords

  • Cancer diagnosis
  • Nucleoli
  • Prostate cancer
  • Symphony staining protocol

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Osunkoya, A. O., Hansel, D. E., Parwani, A. V., Ali, T. Z., Tamas, E. F., Untawale, V. G., Kahane, H., & Epstein, J. I. (2008). The Symphony™ protocol for H&E staining of prostatic adenocarcinoma on needle biopsy: A multicentre analysis of 120 cases. Pathology, 40(5), 450-456. https://doi.org/10.1080/00313020802198127