THE SUBCELLULAR LOCALIZATION OF HISTAMINE AND HISTAMINE METHYLTRANSFERASE IN RAT BRAIN

M. J. Kuhar, K. M. Taylor, S. H. Snyder

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract— We have examined the subcellular localization of histamine and histamine methyl‐transferase (S‐adenosylmethionine: histamine 7V‐methyltransferase; EC 2.1.1.8) in rat brain. The highest levels of histamine and histamine methyltransferase activity were found in the hypothalamus. A large proportion of hypothalamic histamine and histamine methyltransferase activity was found in particles with sedimentation properties in sucrose gradients similar to synaptosomes storing norepinephrine and serotonin. Histamine displayed a bimodal distribution in sucrose gradients. A substantial amount of a tracer dose of [3H]histamine added to hypothalamic homogenates at 4°C was bound to particulate fractions, suggesting that endogenous histamine may redistribute and bind to subcellular fractions during homogenization. The second, lighter peak of histamine in sucrose gradients was thought to be due to histamine that redistributed during homogenization.

Original languageEnglish (US)
Pages (from-to)1515-1527
Number of pages13
JournalJournal of Neurochemistry
Volume18
Issue number8
DOIs
StatePublished - Aug 1971

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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