The structure of GABPα/β: An ETS domain-ankyrin repeat heterodimer bound to DNA

Adrian H. Batchelor, Derek E. Piper, Fabienne Charles De La Brousse, Steven L. McKnight, Cynthia Wolberger

Research output: Contribution to journalArticle

Abstract

GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The α subunit contains a DNA-binding domain that is a member of the ETS family, whereas the α subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABPα/β ETS domain-ankyrin repeat heterodimer bound to DNA was determined at 2.15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an extensive protein- protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABPα ETS domain binds to its core GGA DNA-recognition motif.

Original languageEnglish (US)
Pages (from-to)1037-1041
Number of pages5
JournalScience
Volume279
Issue number5353
DOIs
StatePublished - Feb 13 1998

Fingerprint

GA-Binding Protein Transcription Factor
Ankyrin Repeat
DNA
Proteins
Nucleotide Motifs
ETS Motif

ASJC Scopus subject areas

  • General

Cite this

Batchelor, A. H., Piper, D. E., Charles De La Brousse, F., McKnight, S. L., & Wolberger, C. (1998). The structure of GABPα/β: An ETS domain-ankyrin repeat heterodimer bound to DNA. Science, 279(5353), 1037-1041. https://doi.org/10.1126/science.279.5353.1037

The structure of GABPα/β : An ETS domain-ankyrin repeat heterodimer bound to DNA. / Batchelor, Adrian H.; Piper, Derek E.; Charles De La Brousse, Fabienne; McKnight, Steven L.; Wolberger, Cynthia.

In: Science, Vol. 279, No. 5353, 13.02.1998, p. 1037-1041.

Research output: Contribution to journalArticle

Batchelor, AH, Piper, DE, Charles De La Brousse, F, McKnight, SL & Wolberger, C 1998, 'The structure of GABPα/β: An ETS domain-ankyrin repeat heterodimer bound to DNA', Science, vol. 279, no. 5353, pp. 1037-1041. https://doi.org/10.1126/science.279.5353.1037
Batchelor AH, Piper DE, Charles De La Brousse F, McKnight SL, Wolberger C. The structure of GABPα/β: An ETS domain-ankyrin repeat heterodimer bound to DNA. Science. 1998 Feb 13;279(5353):1037-1041. https://doi.org/10.1126/science.279.5353.1037
Batchelor, Adrian H. ; Piper, Derek E. ; Charles De La Brousse, Fabienne ; McKnight, Steven L. ; Wolberger, Cynthia. / The structure of GABPα/β : An ETS domain-ankyrin repeat heterodimer bound to DNA. In: Science. 1998 ; Vol. 279, No. 5353. pp. 1037-1041.
@article{4fbb426c4f0b42e1880fd761d294874c,
title = "The structure of GABPα/β: An ETS domain-ankyrin repeat heterodimer bound to DNA",
abstract = "GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The α subunit contains a DNA-binding domain that is a member of the ETS family, whereas the α subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABPα/β ETS domain-ankyrin repeat heterodimer bound to DNA was determined at 2.15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an extensive protein- protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABPα ETS domain binds to its core GGA DNA-recognition motif.",
author = "Batchelor, {Adrian H.} and Piper, {Derek E.} and {Charles De La Brousse}, Fabienne and McKnight, {Steven L.} and Cynthia Wolberger",
year = "1998",
month = "2",
day = "13",
doi = "10.1126/science.279.5353.1037",
language = "English (US)",
volume = "279",
pages = "1037--1041",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5353",

}

TY - JOUR

T1 - The structure of GABPα/β

T2 - An ETS domain-ankyrin repeat heterodimer bound to DNA

AU - Batchelor, Adrian H.

AU - Piper, Derek E.

AU - Charles De La Brousse, Fabienne

AU - McKnight, Steven L.

AU - Wolberger, Cynthia

PY - 1998/2/13

Y1 - 1998/2/13

N2 - GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The α subunit contains a DNA-binding domain that is a member of the ETS family, whereas the α subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABPα/β ETS domain-ankyrin repeat heterodimer bound to DNA was determined at 2.15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an extensive protein- protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABPα ETS domain binds to its core GGA DNA-recognition motif.

AB - GA-binding protein (GABP) is a transcriptional regulator composed of two structurally dissimilar subunits. The α subunit contains a DNA-binding domain that is a member of the ETS family, whereas the α subunit contains a series of ankyrin repeats. The crystal structure of a ternary complex containing a GABPα/β ETS domain-ankyrin repeat heterodimer bound to DNA was determined at 2.15 angstrom resolution. The structure shows how an ETS domain protein can recruit a partner protein using both the ETS domain and a carboxyl-terminal extension and provides a view of an extensive protein- protein interface formed by a set of ankyrin repeats. The structure also reveals how the GABPα ETS domain binds to its core GGA DNA-recognition motif.

UR - http://www.scopus.com/inward/record.url?scp=0032512459&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032512459&partnerID=8YFLogxK

U2 - 10.1126/science.279.5353.1037

DO - 10.1126/science.279.5353.1037

M3 - Article

C2 - 9461436

AN - SCOPUS:0032512459

VL - 279

SP - 1037

EP - 1041

JO - Science

JF - Science

SN - 0036-8075

IS - 5353

ER -