The striatal mosaic in primates: Striosomes and matrix are differentially enriched in lonotropic glutamate receptor subunits

Lee J Martin, Craig D. Blackstone, Richard L Huganir, Donald L. Price

Research output: Contribution to journalArticle

Abstract

The cellular and subcellular distributions of the ionotropic α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-preferring glutamate receptor (GluR) in monkey striatum were demonstrated immunocytochemically using anti-peptide antibodies to individual subunits of the AMPA receptor. These antibodies specifically recognize GluR1, GluR4, and an epitope common to GluR2 and GluRS (designated as GluR2/3). On immunoblots, the antibodies detect proteins ranging from 102 to 108 k Da in total homogenates of monkey striatum, hippocampus, and cerebellum. By immunoblotting, GluR1 and GluR2/3 are considerably more abundant than GluR4 in the caudate nucleus. Within the caudate nucleus, putamen, and nucleus accumbens, numerous neuronal perikarya, dendrites, and spines show GluR1 and GluR2/3 immunoreactivities. GluR1- and GluR2/3-enriched striatal neurons have the morphology, transmitter specificity, and distribution of medium-sized (10-20 μm) spiny neurons; large (20-60 μm) round neurons exhibit GluR4 immunoreactivity. GluR1 immunoreactivity, but not GluR2/3 or GluR4 immunoreactivity, is more intense in the ventral striatum (i.e., nucleus accumbens) than in the dorsal striatum, and GluR1 is enriched within dendritic spines in the neuropil of the nucleus accumbens and striosomes in the dorsal striatum. In the caudate nucleus, these patches of dense GluR1 immunoreactivity align with regions low in calcium binding protein immunoreactivity and high in substance P immunoreactivity. Within striosomes, GluR1 immunoreactivity is more abundant than GluR2/3 immunoreactivity; GluR4 immunoreactivity is sparse in striosomes, but the matrix contains large, GluR4-positive cholinergic neurons. This study demonstrates that, within monkey striatum, subunits of ionotropic AMPA GluR have differential distributions within striosomes and matrix. Furthermore, the results suggest that neurons within striatal striosomes and matrix may express different combinations of GluR subunits, thus forming receptors with different channel properties and having consequences that may be relevant physiologically and pathophysiologically. Neurons within these two striatal compartments may have different roles in the synaptic plasticity of motor systems.

Original languageEnglish (US)
Pages (from-to)782-792
Number of pages11
JournalJournal of Neuroscience
Volume13
Issue number2
StatePublished - 1993

Fingerprint

Corpus Striatum
Glutamate Receptors
Primates
Caudate Nucleus
Nucleus Accumbens
Neurons
Haplorhini
AMPA Receptors
Isoxazoles
Ionotropic Glutamate Receptors
Dendritic Spines
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Neuronal Plasticity
Cholinergic Neurons
Calcium-Binding Proteins
Neuropil
Antibodies
Putamen
Substance P
Dendrites

Keywords

  • α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor
  • Caudate nucleus
  • Excitatory amino acids
  • Patch/matrix
  • Putamen
  • Striosome

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The striatal mosaic in primates : Striosomes and matrix are differentially enriched in lonotropic glutamate receptor subunits. / Martin, Lee J; Blackstone, Craig D.; Huganir, Richard L; Price, Donald L.

In: Journal of Neuroscience, Vol. 13, No. 2, 1993, p. 782-792.

Research output: Contribution to journalArticle

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