The stoichiometric production of IL-2 and IFN-γmRNA defines memory T cells that can self-renew after adoptive transfer in humans

Anran Wang, Smita Chandran, Syed A. Shah, Yu Chiu, Biman C. Paria, Tamara Aghamolla, Melissa M. Alvarez-Downing, Chyi Chia Richard Lee, Sanmeet Singh, Thomas Li, Mark E. Dudley, Nicholas P. Restifo, Steven A. Rosenberg, Udai S. Kammula

Research output: Contribution to journalArticle

Abstract

Adoptive immunotherapy using ex vivo-expanded tumor-reactive lymphocytes can mediate durable cancer regression in selected melanoma patients. Analyses of these trials have associated the in vivo engraftment ability of the transferred cells with their antitumor efficacy. Thus, there is intensive clinical interest in the prospective isolation of tumor-specific T cells that can reliably persist after transfer. Animal studies have suggested that central memory CD8+ T cells (TCM) have divergent capabilities including effector differentiation to target antigen and stem cell-like self-renewal that enable long-term survival after adoptive transfer. We sought to isolate human melanoma-specific TCM to define their in vivo fate and function after autologous therapeutic transfer to metastatic patients. To facilitate the high-throughput identification of these rare cells from patients, we report that TCM have a defined stoichiometric production of interleukin-2 (IL-2) and interferon-γ (IFN-γ) mRNA after antigen stimulation. Melanoma-specific T cells screened for high relative IL-2 production had a TCM phenotype and superior in vitro proliferative capacity compared to cells with low IL-2 production. To investigate in vivo effector function and self-renewal capability, we allowed melanoma-specific TCM to undergo in vitro expansion and differentiation into lytic effector clones and then adoptively transferred them back into their hosts. These clones targeted skin melanocytes in all five patients and persisted long term and reacquired parental TCM attributes in four patients after transfer. These findings demonstrate the favorable engraftment fitness for human TCM-derived clones, but further efforts to improve their antitumor efficacy are still necessary.

Original languageEnglish (US)
Article number149ra120
JournalScience Translational Medicine
Volume4
Issue number149
DOIs
StatePublished - Aug 29 2012
Externally publishedYes

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Adoptive Transfer
Interferons
Interleukin-2
Melanoma
T-Lymphocytes
Messenger RNA
Clone Cells
Adoptive Immunotherapy
Antigens
Patient Transfer
Neoplasms
Melanocytes
Lymphocytes
Phenotype
Skin
Survival
In Vitro Techniques
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The stoichiometric production of IL-2 and IFN-γmRNA defines memory T cells that can self-renew after adoptive transfer in humans. / Wang, Anran; Chandran, Smita; Shah, Syed A.; Chiu, Yu; Paria, Biman C.; Aghamolla, Tamara; Alvarez-Downing, Melissa M.; Lee, Chyi Chia Richard; Singh, Sanmeet; Li, Thomas; Dudley, Mark E.; Restifo, Nicholas P.; Rosenberg, Steven A.; Kammula, Udai S.

In: Science Translational Medicine, Vol. 4, No. 149, 149ra120, 29.08.2012.

Research output: Contribution to journalArticle

Wang, A, Chandran, S, Shah, SA, Chiu, Y, Paria, BC, Aghamolla, T, Alvarez-Downing, MM, Lee, CCR, Singh, S, Li, T, Dudley, ME, Restifo, NP, Rosenberg, SA & Kammula, US 2012, 'The stoichiometric production of IL-2 and IFN-γmRNA defines memory T cells that can self-renew after adoptive transfer in humans', Science Translational Medicine, vol. 4, no. 149, 149ra120. https://doi.org/10.1126/scitranslmed.3004306
Wang, Anran ; Chandran, Smita ; Shah, Syed A. ; Chiu, Yu ; Paria, Biman C. ; Aghamolla, Tamara ; Alvarez-Downing, Melissa M. ; Lee, Chyi Chia Richard ; Singh, Sanmeet ; Li, Thomas ; Dudley, Mark E. ; Restifo, Nicholas P. ; Rosenberg, Steven A. ; Kammula, Udai S. / The stoichiometric production of IL-2 and IFN-γmRNA defines memory T cells that can self-renew after adoptive transfer in humans. In: Science Translational Medicine. 2012 ; Vol. 4, No. 149.
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AU - Alvarez-Downing, Melissa M.

AU - Lee, Chyi Chia Richard

AU - Singh, Sanmeet

AU - Li, Thomas

AU - Dudley, Mark E.

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AU - Rosenberg, Steven A.

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