The specificity of the cAMP receptor mediating activation of adenylate cyclase in Dictyostelium discoideum

Anne Theibert, Maria Palmisano, Bernd Jastorff, Peter N Devreotes

Research output: Contribution to journalArticle

Abstract

In Dictyostelium discoideumamoebae, binding of cyclic AMP (cAMP) to surface receptors elicits numerous responses including chemotaxis, cyclic GMP (cGMP) accumulation, and activation of adenylate cyclase. The specificity of the surface cAMP receptor which mediates activation of adenylate cyclase and cAMP secretion was determined by testing the relative effectiveness of a series of 10 cAMP analogs. Each of the 10 analogs elicited cAMP secretion, chemotaxis, and cGMP accumulation in the same dose range. The order of potency for eliciting these responses (cAMP > 2′-H-cAMP >N1-O-cAMP > cAMPS(Sp) > 6-Cl-cAMP > cAMPN(CH3)2(Sp) > 3′-NH-cAMP > 8-Br-cAMP > cAMPS(Rp) > cAMPN(CH3)2(Rp)) matches that for binding to the major cell surface cAMP binding sites and differs from that of the cell surface phosphodiesterase and the major intracellular cAMP binding protein.

Original languageEnglish (US)
Pages (from-to)529-533
Number of pages5
JournalDevelopmental Biology
Volume114
Issue number2
DOIs
StatePublished - 1986

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Cyclic AMP Receptors
Dictyostelium
Adenylyl Cyclases
Cyclic AMP
Cyclic GMP
Chemotaxis
8-Bromo Cyclic Adenosine Monophosphate
Phosphoric Diester Hydrolases
Carrier Proteins

ASJC Scopus subject areas

  • Developmental Biology

Cite this

The specificity of the cAMP receptor mediating activation of adenylate cyclase in Dictyostelium discoideum. / Theibert, Anne; Palmisano, Maria; Jastorff, Bernd; Devreotes, Peter N.

In: Developmental Biology, Vol. 114, No. 2, 1986, p. 529-533.

Research output: Contribution to journalArticle

Theibert, Anne ; Palmisano, Maria ; Jastorff, Bernd ; Devreotes, Peter N. / The specificity of the cAMP receptor mediating activation of adenylate cyclase in Dictyostelium discoideum. In: Developmental Biology. 1986 ; Vol. 114, No. 2. pp. 529-533.
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