TY - JOUR
T1 - The specificity of the cAMP receptor mediating activation of adenylate cyclase in Dictyostelium discoideum
AU - Theibert, Anne
AU - Palmisano, Maria
AU - Jastorff, Bernd
AU - Devreotes, Peter
N1 - Funding Information:
This research was supported by NIH Grant GM 28007. Dr. Devreotes is an American Heart Association Established Investigator.
PY - 1986/4
Y1 - 1986/4
N2 - In Dictyostelium discoideumamoebae, binding of cyclic AMP (cAMP) to surface receptors elicits numerous responses including chemotaxis, cyclic GMP (cGMP) accumulation, and activation of adenylate cyclase. The specificity of the surface cAMP receptor which mediates activation of adenylate cyclase and cAMP secretion was determined by testing the relative effectiveness of a series of 10 cAMP analogs. Each of the 10 analogs elicited cAMP secretion, chemotaxis, and cGMP accumulation in the same dose range. The order of potency for eliciting these responses (cAMP > 2′-H-cAMP >N1-O-cAMP > cAMPS(Sp) > 6-Cl-cAMP > cAMPN(CH3)2(Sp) > 3′-NH-cAMP > 8-Br-cAMP > cAMPS(Rp) > cAMPN(CH3)2(Rp)) matches that for binding to the major cell surface cAMP binding sites and differs from that of the cell surface phosphodiesterase and the major intracellular cAMP binding protein.
AB - In Dictyostelium discoideumamoebae, binding of cyclic AMP (cAMP) to surface receptors elicits numerous responses including chemotaxis, cyclic GMP (cGMP) accumulation, and activation of adenylate cyclase. The specificity of the surface cAMP receptor which mediates activation of adenylate cyclase and cAMP secretion was determined by testing the relative effectiveness of a series of 10 cAMP analogs. Each of the 10 analogs elicited cAMP secretion, chemotaxis, and cGMP accumulation in the same dose range. The order of potency for eliciting these responses (cAMP > 2′-H-cAMP >N1-O-cAMP > cAMPS(Sp) > 6-Cl-cAMP > cAMPN(CH3)2(Sp) > 3′-NH-cAMP > 8-Br-cAMP > cAMPS(Rp) > cAMPN(CH3)2(Rp)) matches that for binding to the major cell surface cAMP binding sites and differs from that of the cell surface phosphodiesterase and the major intracellular cAMP binding protein.
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U2 - 10.1016/0012-1606(86)90216-2
DO - 10.1016/0012-1606(86)90216-2
M3 - Article
C2 - 3007244
AN - SCOPUS:0022575305
SN - 0012-1606
VL - 114
SP - 529
EP - 533
JO - Developmental biology
JF - Developmental biology
IS - 2
ER -