The SOCS Box Protein STOPS Is Required for Phototransduction through Its Effects on Phospholipase C

Tao Wang, Xiaoyue Wang, Qiang Xie, Craig Montell

Research output: Contribution to journalArticle

Abstract

Phosphoinositide-specific phospholipase C (PLC) isozymes play roles in a diversity of processes including Drosophila phototransduction. In fly photoreceptor cells, the PLCβ encoded by norpA is critical for activation of TRP channels. Here, we describe a PLCβ regulator, STOPS, which encodes a SOCS box protein. Mutation of stops resulted in a reduced concentration of NORPA and a defect in stopping signaling following cessation of the light stimulus. NORPA has been proposed to have dual roles as a PLC- and GTPase-activating protein (GAP). We found that the slow termination resulting from expressing low levels of wild-type NORPA was suppressed by addition of normal amounts of an altered NORPA, which had wild-type GAP activity, but no PLC activity. STOPS is the first protein identified that specifically regulates PLCβ protein concentration. Moreover, this work demonstrates that a PLCβ derivative that does not promote TRP channel activation, still contributes to signaling in vivo.

Original languageEnglish (US)
Pages (from-to)56-68
Number of pages13
JournalNeuron
Volume57
Issue number1
DOIs
StatePublished - Jan 10 2008

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Suppressor of Cytokine Signaling Proteins
Type C Phospholipases
GTPase-Activating Proteins
Light Signal Transduction
Phosphoinositide Phospholipase C
Photoreceptor Cells
Diptera
Isoenzymes
Drosophila
Proteins
Light
Mutation

Keywords

  • MOLNEURO
  • PROTEINS
  • SIGNALING

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The SOCS Box Protein STOPS Is Required for Phototransduction through Its Effects on Phospholipase C. / Wang, Tao; Wang, Xiaoyue; Xie, Qiang; Montell, Craig.

In: Neuron, Vol. 57, No. 1, 10.01.2008, p. 56-68.

Research output: Contribution to journalArticle

Wang, Tao ; Wang, Xiaoyue ; Xie, Qiang ; Montell, Craig. / The SOCS Box Protein STOPS Is Required for Phototransduction through Its Effects on Phospholipase C. In: Neuron. 2008 ; Vol. 57, No. 1. pp. 56-68.
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