The significance of enteroaggregative Escherichia coli in the etiology of hospitalized diarrhoea in Calcutta, India and the demonstration of a new honey-combed pattern of aggregative adherence

Mousumi Paul, Teizo Tsukamoto, Asit R. Ghosh, S. K. Bhattacharya, B. Manna, S. Chakrabarti, G. Balakrish Nair, David Allen Sack, Dipak Sen, Yoshifumi Takeda

Research output: Contribution to journalArticle

Abstract

Previous studies have identified enteroadherent Escherichia coli that exhibit localized adherence, diffuse adherence and atypical diffuse adherence as diarrhoeagenic agents associated with infantile diarrhoea in Calcutta, India. In this study, a DNA probe specific for enteroaggregatove adherence was used to determine the etiological significance of enteroaggregative E. coli in the causation of diarrhoea. From a total of 330 strains of E. coli recovered from 159 cases of acute secretory diarrhoea and 174 cases of invasive diarrhoea, 20 strains hybridized with the probe, whereas of the 25 E. coli strains recovered from 25 healthy controls only 1 strain hybridized with the probe. Of the 21 probe positive strains, 19 adhered to HeLa cells in the typical stacked-brick pattern while 2 strains recovered from 2 cases of secretory diarrhoea adhered to the glass surface in a hitherto undescribed formation which we have termed, based on the appearance, as the honey-comb pattern. The enteroaggregative E. coli strains identified in this study did not produce any conventional enterotoxins and were significantly associated with patients with secretory diarrhoea (10.7%) than with invasive diarrhoea (1.7%). The results of this study indicate that enteroaggregative E. coli play a causal role in acute secretory diarrhoea in this part of the world which lends credence to the involvement of a potent toxin in the pathogenesis of EAggEC mediated infections.

Original languageEnglish (US)
Pages (from-to)319-325
Number of pages7
JournalFEMS Microbiology Letters
Volume117
Issue number3
StatePublished - Apr 15 1994

Fingerprint

Honey
India
Diarrhea
Escherichia coli
Infantile Diarrhea
Comb and Wattles
Enterotoxins
DNA Probes
HeLa Cells
Causality
Glass
Infection

Keywords

  • DNA probe
  • Enteroaggregative Escherichia coli
  • Honey-comb pattern
  • Secretory diarrhoea

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

The significance of enteroaggregative Escherichia coli in the etiology of hospitalized diarrhoea in Calcutta, India and the demonstration of a new honey-combed pattern of aggregative adherence. / Paul, Mousumi; Tsukamoto, Teizo; Ghosh, Asit R.; Bhattacharya, S. K.; Manna, B.; Chakrabarti, S.; Balakrish Nair, G.; Sack, David Allen; Sen, Dipak; Takeda, Yoshifumi.

In: FEMS Microbiology Letters, Vol. 117, No. 3, 15.04.1994, p. 319-325.

Research output: Contribution to journalArticle

Paul, M, Tsukamoto, T, Ghosh, AR, Bhattacharya, SK, Manna, B, Chakrabarti, S, Balakrish Nair, G, Sack, DA, Sen, D & Takeda, Y 1994, 'The significance of enteroaggregative Escherichia coli in the etiology of hospitalized diarrhoea in Calcutta, India and the demonstration of a new honey-combed pattern of aggregative adherence', FEMS Microbiology Letters, vol. 117, no. 3, pp. 319-325.
Paul, Mousumi ; Tsukamoto, Teizo ; Ghosh, Asit R. ; Bhattacharya, S. K. ; Manna, B. ; Chakrabarti, S. ; Balakrish Nair, G. ; Sack, David Allen ; Sen, Dipak ; Takeda, Yoshifumi. / The significance of enteroaggregative Escherichia coli in the etiology of hospitalized diarrhoea in Calcutta, India and the demonstration of a new honey-combed pattern of aggregative adherence. In: FEMS Microbiology Letters. 1994 ; Vol. 117, No. 3. pp. 319-325.
@article{1f2afb5f4d0f4111b122110ee86f9f36,
title = "The significance of enteroaggregative Escherichia coli in the etiology of hospitalized diarrhoea in Calcutta, India and the demonstration of a new honey-combed pattern of aggregative adherence",
abstract = "Previous studies have identified enteroadherent Escherichia coli that exhibit localized adherence, diffuse adherence and atypical diffuse adherence as diarrhoeagenic agents associated with infantile diarrhoea in Calcutta, India. In this study, a DNA probe specific for enteroaggregatove adherence was used to determine the etiological significance of enteroaggregative E. coli in the causation of diarrhoea. From a total of 330 strains of E. coli recovered from 159 cases of acute secretory diarrhoea and 174 cases of invasive diarrhoea, 20 strains hybridized with the probe, whereas of the 25 E. coli strains recovered from 25 healthy controls only 1 strain hybridized with the probe. Of the 21 probe positive strains, 19 adhered to HeLa cells in the typical stacked-brick pattern while 2 strains recovered from 2 cases of secretory diarrhoea adhered to the glass surface in a hitherto undescribed formation which we have termed, based on the appearance, as the honey-comb pattern. The enteroaggregative E. coli strains identified in this study did not produce any conventional enterotoxins and were significantly associated with patients with secretory diarrhoea (10.7{\%}) than with invasive diarrhoea (1.7{\%}). The results of this study indicate that enteroaggregative E. coli play a causal role in acute secretory diarrhoea in this part of the world which lends credence to the involvement of a potent toxin in the pathogenesis of EAggEC mediated infections.",
keywords = "DNA probe, Enteroaggregative Escherichia coli, Honey-comb pattern, Secretory diarrhoea",
author = "Mousumi Paul and Teizo Tsukamoto and Ghosh, {Asit R.} and Bhattacharya, {S. K.} and B. Manna and S. Chakrabarti and {Balakrish Nair}, G. and Sack, {David Allen} and Dipak Sen and Yoshifumi Takeda",
year = "1994",
month = "4",
day = "15",
language = "English (US)",
volume = "117",
pages = "319--325",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - The significance of enteroaggregative Escherichia coli in the etiology of hospitalized diarrhoea in Calcutta, India and the demonstration of a new honey-combed pattern of aggregative adherence

AU - Paul, Mousumi

AU - Tsukamoto, Teizo

AU - Ghosh, Asit R.

AU - Bhattacharya, S. K.

AU - Manna, B.

AU - Chakrabarti, S.

AU - Balakrish Nair, G.

AU - Sack, David Allen

AU - Sen, Dipak

AU - Takeda, Yoshifumi

PY - 1994/4/15

Y1 - 1994/4/15

N2 - Previous studies have identified enteroadherent Escherichia coli that exhibit localized adherence, diffuse adherence and atypical diffuse adherence as diarrhoeagenic agents associated with infantile diarrhoea in Calcutta, India. In this study, a DNA probe specific for enteroaggregatove adherence was used to determine the etiological significance of enteroaggregative E. coli in the causation of diarrhoea. From a total of 330 strains of E. coli recovered from 159 cases of acute secretory diarrhoea and 174 cases of invasive diarrhoea, 20 strains hybridized with the probe, whereas of the 25 E. coli strains recovered from 25 healthy controls only 1 strain hybridized with the probe. Of the 21 probe positive strains, 19 adhered to HeLa cells in the typical stacked-brick pattern while 2 strains recovered from 2 cases of secretory diarrhoea adhered to the glass surface in a hitherto undescribed formation which we have termed, based on the appearance, as the honey-comb pattern. The enteroaggregative E. coli strains identified in this study did not produce any conventional enterotoxins and were significantly associated with patients with secretory diarrhoea (10.7%) than with invasive diarrhoea (1.7%). The results of this study indicate that enteroaggregative E. coli play a causal role in acute secretory diarrhoea in this part of the world which lends credence to the involvement of a potent toxin in the pathogenesis of EAggEC mediated infections.

AB - Previous studies have identified enteroadherent Escherichia coli that exhibit localized adherence, diffuse adherence and atypical diffuse adherence as diarrhoeagenic agents associated with infantile diarrhoea in Calcutta, India. In this study, a DNA probe specific for enteroaggregatove adherence was used to determine the etiological significance of enteroaggregative E. coli in the causation of diarrhoea. From a total of 330 strains of E. coli recovered from 159 cases of acute secretory diarrhoea and 174 cases of invasive diarrhoea, 20 strains hybridized with the probe, whereas of the 25 E. coli strains recovered from 25 healthy controls only 1 strain hybridized with the probe. Of the 21 probe positive strains, 19 adhered to HeLa cells in the typical stacked-brick pattern while 2 strains recovered from 2 cases of secretory diarrhoea adhered to the glass surface in a hitherto undescribed formation which we have termed, based on the appearance, as the honey-comb pattern. The enteroaggregative E. coli strains identified in this study did not produce any conventional enterotoxins and were significantly associated with patients with secretory diarrhoea (10.7%) than with invasive diarrhoea (1.7%). The results of this study indicate that enteroaggregative E. coli play a causal role in acute secretory diarrhoea in this part of the world which lends credence to the involvement of a potent toxin in the pathogenesis of EAggEC mediated infections.

KW - DNA probe

KW - Enteroaggregative Escherichia coli

KW - Honey-comb pattern

KW - Secretory diarrhoea

UR - http://www.scopus.com/inward/record.url?scp=0028179315&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028179315&partnerID=8YFLogxK

M3 - Article

C2 - 8200507

AN - SCOPUS:0028179315

VL - 117

SP - 319

EP - 325

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 3

ER -