The signaling pathway for aldosterone-induced mitochondrial production of superoxide anion in the myocardium

M. B. Nolly, C. I. Caldiz, A. M. Yeves, M. C. Villa-Abrille, P. E. Morgan, Nicolas Amado Mondaca, Enrique L. Portiansky, G. E. Chiappe de Cingolani, H. E. Cingolani, I. L. Ennis

Research output: Contribution to journalArticle

Abstract

Mineralocorticoid receptor (MR) antagonists decrease morbidity and mortality in heart failure patients for whom oxidative stress is usual; however, the underlying mechanism for this protection is unclear. Since aldosterone stimulates reactive oxygen species (ROS) production in several tissues, we explored its effect and the intracellular pathway involved in the rat myocardium. Aldosterone dose-dependently increased O2- production in myocardial slices. At 10nmol/L, aldosterone increased O2- to 165±8.8% of control, an effect prevented not only by the MR antagonists eplerenone and spironolactone (107±7.8 and 103±5.3%, respectively) but also by AG1478 (105±8.0%), antagonist of the EGF receptor (EGFR). Similar results were obtained by silencing MR expression through the direct intramyocardial injection of a lentivirus coding for a siRNA against the MR. The aldosterone effect on O2- production was mimicked by the mKATP channel opener diazoxide and blocked by preventing its opening with 5-HD and glibenclamide, implicating the mitochondria as the source of O2-. Inhibiting the respiratory chain with rotenone or mitochondrial permeability transition (MPT) with cyclosporine A or bongkrekic acid also canceled aldosterone-induced O2- production. In addition, aldosterone effect depended on NADPH oxidase and phosphoinositide 3-kinase activation, as apocynin and wortmannin, respectively, inhibited it. EGF (0.1μg/mL) similarly increased O2-, although in this case MR antagonists had no effect, suggesting that EGFR transactivation occurred downstream from MR activation. Inhibition of mKATP channels, the respiratory chain, or MPT did not prevent Akt phosphorylation, supporting that it happened upstream of the mitochondria. Importantly, cardiomyocytes were confirmed as a source of aldosterone induced mitochondrial ROS production in experiments performed in isolated cardiac myocytes.These results allow us to speculate that the beneficial effects of MR antagonists in heart failure may be related to a decrease in oxidative stress.

Original languageEnglish (US)
Pages (from-to)60-68
Number of pages9
JournalJournal of Molecular and Cellular Cardiology
Volume67
DOIs
StatePublished - Feb 2014
Externally publishedYes

Fingerprint

Aldosterone
Superoxides
Myocardium
Mineralocorticoid Receptor Antagonists
Mineralocorticoid Receptors
Electron Transport
Epidermal Growth Factor Receptor
Cardiac Myocytes
Permeability
Reactive Oxygen Species
Mitochondria
Bongkrekic Acid
Oxidative Stress
Heart Failure
Diazoxide
Rotenone
Lentivirus
Spironolactone
1-Phosphatidylinositol 4-Kinase
Glyburide

Keywords

  • Mineralocorticoid receptor
  • Oxidative stress
  • SiRNA
  • Transactivation

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Nolly, M. B., Caldiz, C. I., Yeves, A. M., Villa-Abrille, M. C., Morgan, P. E., Amado Mondaca, N., ... Ennis, I. L. (2014). The signaling pathway for aldosterone-induced mitochondrial production of superoxide anion in the myocardium. Journal of Molecular and Cellular Cardiology, 67, 60-68. https://doi.org/10.1016/j.yjmcc.2013.12.004

The signaling pathway for aldosterone-induced mitochondrial production of superoxide anion in the myocardium. / Nolly, M. B.; Caldiz, C. I.; Yeves, A. M.; Villa-Abrille, M. C.; Morgan, P. E.; Amado Mondaca, Nicolas; Portiansky, Enrique L.; Chiappe de Cingolani, G. E.; Cingolani, H. E.; Ennis, I. L.

In: Journal of Molecular and Cellular Cardiology, Vol. 67, 02.2014, p. 60-68.

Research output: Contribution to journalArticle

Nolly, MB, Caldiz, CI, Yeves, AM, Villa-Abrille, MC, Morgan, PE, Amado Mondaca, N, Portiansky, EL, Chiappe de Cingolani, GE, Cingolani, HE & Ennis, IL 2014, 'The signaling pathway for aldosterone-induced mitochondrial production of superoxide anion in the myocardium', Journal of Molecular and Cellular Cardiology, vol. 67, pp. 60-68. https://doi.org/10.1016/j.yjmcc.2013.12.004
Nolly, M. B. ; Caldiz, C. I. ; Yeves, A. M. ; Villa-Abrille, M. C. ; Morgan, P. E. ; Amado Mondaca, Nicolas ; Portiansky, Enrique L. ; Chiappe de Cingolani, G. E. ; Cingolani, H. E. ; Ennis, I. L. / The signaling pathway for aldosterone-induced mitochondrial production of superoxide anion in the myocardium. In: Journal of Molecular and Cellular Cardiology. 2014 ; Vol. 67. pp. 60-68.
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abstract = "Mineralocorticoid receptor (MR) antagonists decrease morbidity and mortality in heart failure patients for whom oxidative stress is usual; however, the underlying mechanism for this protection is unclear. Since aldosterone stimulates reactive oxygen species (ROS) production in several tissues, we explored its effect and the intracellular pathway involved in the rat myocardium. Aldosterone dose-dependently increased O2- production in myocardial slices. At 10nmol/L, aldosterone increased O2- to 165±8.8{\%} of control, an effect prevented not only by the MR antagonists eplerenone and spironolactone (107±7.8 and 103±5.3{\%}, respectively) but also by AG1478 (105±8.0{\%}), antagonist of the EGF receptor (EGFR). Similar results were obtained by silencing MR expression through the direct intramyocardial injection of a lentivirus coding for a siRNA against the MR. The aldosterone effect on O2- production was mimicked by the mKATP channel opener diazoxide and blocked by preventing its opening with 5-HD and glibenclamide, implicating the mitochondria as the source of O2-. Inhibiting the respiratory chain with rotenone or mitochondrial permeability transition (MPT) with cyclosporine A or bongkrekic acid also canceled aldosterone-induced O2- production. In addition, aldosterone effect depended on NADPH oxidase and phosphoinositide 3-kinase activation, as apocynin and wortmannin, respectively, inhibited it. EGF (0.1μg/mL) similarly increased O2-, although in this case MR antagonists had no effect, suggesting that EGFR transactivation occurred downstream from MR activation. Inhibition of mKATP channels, the respiratory chain, or MPT did not prevent Akt phosphorylation, supporting that it happened upstream of the mitochondria. Importantly, cardiomyocytes were confirmed as a source of aldosterone induced mitochondrial ROS production in experiments performed in isolated cardiac myocytes.These results allow us to speculate that the beneficial effects of MR antagonists in heart failure may be related to a decrease in oxidative stress.",
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AU - Morgan, P. E.

AU - Amado Mondaca, Nicolas

AU - Portiansky, Enrique L.

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N2 - Mineralocorticoid receptor (MR) antagonists decrease morbidity and mortality in heart failure patients for whom oxidative stress is usual; however, the underlying mechanism for this protection is unclear. Since aldosterone stimulates reactive oxygen species (ROS) production in several tissues, we explored its effect and the intracellular pathway involved in the rat myocardium. Aldosterone dose-dependently increased O2- production in myocardial slices. At 10nmol/L, aldosterone increased O2- to 165±8.8% of control, an effect prevented not only by the MR antagonists eplerenone and spironolactone (107±7.8 and 103±5.3%, respectively) but also by AG1478 (105±8.0%), antagonist of the EGF receptor (EGFR). Similar results were obtained by silencing MR expression through the direct intramyocardial injection of a lentivirus coding for a siRNA against the MR. The aldosterone effect on O2- production was mimicked by the mKATP channel opener diazoxide and blocked by preventing its opening with 5-HD and glibenclamide, implicating the mitochondria as the source of O2-. Inhibiting the respiratory chain with rotenone or mitochondrial permeability transition (MPT) with cyclosporine A or bongkrekic acid also canceled aldosterone-induced O2- production. In addition, aldosterone effect depended on NADPH oxidase and phosphoinositide 3-kinase activation, as apocynin and wortmannin, respectively, inhibited it. EGF (0.1μg/mL) similarly increased O2-, although in this case MR antagonists had no effect, suggesting that EGFR transactivation occurred downstream from MR activation. Inhibition of mKATP channels, the respiratory chain, or MPT did not prevent Akt phosphorylation, supporting that it happened upstream of the mitochondria. Importantly, cardiomyocytes were confirmed as a source of aldosterone induced mitochondrial ROS production in experiments performed in isolated cardiac myocytes.These results allow us to speculate that the beneficial effects of MR antagonists in heart failure may be related to a decrease in oxidative stress.

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