The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase

Lucas Vicuña, David E. Strochlic, Alban Latremoliere, Kiran Kumar Bali, Manuela Simonetti, Dewi Husainie, Sandra Prokosch, Priscilla Riva, Robert S. Griffin, Christian Njoo, Stefanie Gehrig, Marcus A. Mall, Bernd Arnold, Marshall Devor, Clifford J. Woolf, Stephen D. Liberles, Michael Costigan, Rohini Kuner

Research output: Contribution to journalArticle

Abstract

Neuropathic pain is a major, intractable clinical problem and its pathophysiology is not well understood. Although recent gene expression profiling studies have enabled the identification of novel targets for pain therapy, classical study designs provide unclear results owing to the differential expression of hundreds of genes across sham and nerve-injured groups, which can be difficult to validate, particularly with respect to the specificity of pain modulation. To circumvent this, we used two outbred lines of rats, which are genetically similar except for being genetically segregated as a result of selective breeding for differences in neuropathic pain hypersensitivity. SerpinA3N, a serine protease inhibitor, was upregulated in the dorsal root ganglia (DRG) after nerve injury, which was further validated for its mouse homolog. Mice lacking SerpinA3N developed more neuropathic mechanical allodynia than wild-type (WT) mice, and exogenous delivery of SerpinA3N attenuated mechanical allodynia in WT mice. T lymphocytes infiltrate the DRG after nerve injury and release leukocyte elastase (LE), which was inhibited by SerpinA3N derived from DRG neurons. Genetic loss of LE or exogenous application of a LE inhibitor (Sivelastat) in WT mice attenuated neuropathic mechanical allodynia. Overall, we reveal a novel and clinically relevant role for a member of the serpin superfamily and a leukocyte elastase and crosstalk between neurons and T cells in the modulation of neuropathic pain.

Original languageEnglish (US)
Pages (from-to)518-523
Number of pages6
JournalNature Medicine
Volume21
Issue number5
DOIs
StatePublished - May 1 2015
Externally publishedYes

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Leukocyte Elastase
Serine Proteinase Inhibitors
T-cells
Neuralgia
Hyperalgesia
T-Lymphocytes
Spinal Ganglia
Neurons
Modulation
Serpins
Pain
Crosstalk
Gene expression
Wounds and Injuries
Rats
Gene Expression Profiling
Genes
Hypersensitivity
Gene Expression

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase. / Vicuña, Lucas; Strochlic, David E.; Latremoliere, Alban; Bali, Kiran Kumar; Simonetti, Manuela; Husainie, Dewi; Prokosch, Sandra; Riva, Priscilla; Griffin, Robert S.; Njoo, Christian; Gehrig, Stefanie; Mall, Marcus A.; Arnold, Bernd; Devor, Marshall; Woolf, Clifford J.; Liberles, Stephen D.; Costigan, Michael; Kuner, Rohini.

In: Nature Medicine, Vol. 21, No. 5, 01.05.2015, p. 518-523.

Research output: Contribution to journalArticle

Vicuña, L, Strochlic, DE, Latremoliere, A, Bali, KK, Simonetti, M, Husainie, D, Prokosch, S, Riva, P, Griffin, RS, Njoo, C, Gehrig, S, Mall, MA, Arnold, B, Devor, M, Woolf, CJ, Liberles, SD, Costigan, M & Kuner, R 2015, 'The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase', Nature Medicine, vol. 21, no. 5, pp. 518-523. https://doi.org/10.1038/nm.3852
Vicuña, Lucas ; Strochlic, David E. ; Latremoliere, Alban ; Bali, Kiran Kumar ; Simonetti, Manuela ; Husainie, Dewi ; Prokosch, Sandra ; Riva, Priscilla ; Griffin, Robert S. ; Njoo, Christian ; Gehrig, Stefanie ; Mall, Marcus A. ; Arnold, Bernd ; Devor, Marshall ; Woolf, Clifford J. ; Liberles, Stephen D. ; Costigan, Michael ; Kuner, Rohini. / The serine protease inhibitor SerpinA3N attenuates neuropathic pain by inhibiting T cell-derived leukocyte elastase. In: Nature Medicine. 2015 ; Vol. 21, No. 5. pp. 518-523.
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