The Search for Plasmodium falciparum histidine-rich protein 2/3 Deletions in Zambia and Implications for Plasmodium falciparum histidine-rich protein 2-Based Rapid Diagnostic Tests

Tamaki Kobayashi, Jay Sikalima, Jonathan B. Parr, Mike Chaponda, Jennifer Claire Stevenson, Philip E Thuma, Modest Mulenga, Steven R. Meshnick, William J Moss, The Southern For The Southern

Research output: Contribution to journalArticle

Abstract

We attempted to identify Plasmodium falciparum histidine-rich protein 2/3 (pfhrp2/3) deletions among rapid diagnostic test (RDT)-negative but PCR- or microscopy-positive P. falciparum-infected individuals in areas of low transmission (Choma District, 2009-2011) and high transmission (Nchelenge District, 2015-2017) in Zambia. Through community-based surveys, 5,167 participants were screened at 1,147 households by P. falciparum histidine-rich protein 2 (PfHRP2)-based RDTs. Slides were made and dried blood spots were obtained for molecular analysis. Of 28 samples with detectable P. falciparum DNA, none from Nchelenge District were pfhrp2/3 negative. All eight samples from Choma District had detectable pfhrp3 genes, but pfhrp2 was undetectable in three. DNA concentrations of pfhrp2-negative samples were low (< 0.001 ng/μL). These findings suggest that PfHRP2-based RDTs remain effective tools for malaria diagnosis in Nchelenge District, but further study is warranted to understand the potential for pfhrp2/3 deletions in southern Zambia where malaria transmission declined over the past decade.

Original languageEnglish (US)
Pages (from-to)842-845
Number of pages4
JournalThe American journal of tropical medicine and hygiene
Volume100
Issue number4
DOIs
StatePublished - Apr 1 2019

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Zambia
Plasmodium falciparum
Routine Diagnostic Tests
Malaria
DNA
Microscopy
Polymerase Chain Reaction
Genes
histidine-rich proteins
Plasmodium falciparum HRP3 protein
Plasmodium falciparum HRP-2 antigen

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

The Search for Plasmodium falciparum histidine-rich protein 2/3 Deletions in Zambia and Implications for Plasmodium falciparum histidine-rich protein 2-Based Rapid Diagnostic Tests. / Kobayashi, Tamaki; Sikalima, Jay; Parr, Jonathan B.; Chaponda, Mike; Stevenson, Jennifer Claire; Thuma, Philip E; Mulenga, Modest; Meshnick, Steven R.; Moss, William J; For The Southern, The Southern.

In: The American journal of tropical medicine and hygiene, Vol. 100, No. 4, 01.04.2019, p. 842-845.

Research output: Contribution to journalArticle

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