TY - JOUR
T1 - The search for plasmodium falciparum histidine-rich protein 2/3 deletions in Zambia and implications for plasmodium falciparum histidine-rich protein 2-based rapid diagnostic tests
AU - Kobayashi, Tamaki
AU - Sikalima, Jay
AU - Parr, Jonathan B.
AU - Chaponda, Mike
AU - Stevenson, Jennifer C.
AU - Thuma, Philip E.
AU - Mulenga, Modest
AU - Meshnick, Steven R.
AU - Moss, William J.
N1 - Funding Information:
Acknowledgments: This work was supported by the Johns Hopkins Malaria Research Institute, the Bloomberg Family Foundation, and the Division of Microbiology and Infectious Diseases, National Institutes of Allergies and Infectious Diseases, National Institutes of Health as part of the International Centers of Excellence for Malaria Research (U19 AI089680). We thank the field teams and laboratory staff at Macha Research Trust and the Tropical Disease Research Centre. Most importantly, we thank the residents of the Macha community and the Nchelenge community who participated in this study.
Publisher Copyright:
© 2019 by The American Society of Tropical Medicine and Hygiene.
PY - 2019
Y1 - 2019
N2 - Weattempted to identify Plasmodium falciparum histidine-rich protein 2/3 (pfhrp2/3) deletions among rapid diagnostic test (RDT)-negative but PCR- or microscopy-positive P. falciparum-infected individuals in areas of low transmission (Choma District, 2009-2011) and high transmission (Nchelenge District, 2015-2017) in Zambia. Through community-based surveys, 5,167 participants were screened at 1,147 households by P. falciparum histidine-rich protein 2 (PfHRP2)-based RDTs. Slides were made and dried blood spots were obtained for molecular analysis. Of 28 samples with detectable P. falciparum DNA, none from Nchelenge District were pfhrp2/3 negative. All eight samples from Choma District had detectable pfhrp3 genes, but pfhrp2 was undetectable in three. DNA concentrations of pfhrp2-negative samples were low (< 0.001 ng/μL). These findings suggest that PfHRP2-based RDTs remain effective tools for malaria diagnosis in Nchelenge District, but further study is warranted to understand the potential for pfhrp2/3 deletions in southern Zambia where malaria transmission declined over the past decade.
AB - Weattempted to identify Plasmodium falciparum histidine-rich protein 2/3 (pfhrp2/3) deletions among rapid diagnostic test (RDT)-negative but PCR- or microscopy-positive P. falciparum-infected individuals in areas of low transmission (Choma District, 2009-2011) and high transmission (Nchelenge District, 2015-2017) in Zambia. Through community-based surveys, 5,167 participants were screened at 1,147 households by P. falciparum histidine-rich protein 2 (PfHRP2)-based RDTs. Slides were made and dried blood spots were obtained for molecular analysis. Of 28 samples with detectable P. falciparum DNA, none from Nchelenge District were pfhrp2/3 negative. All eight samples from Choma District had detectable pfhrp3 genes, but pfhrp2 was undetectable in three. DNA concentrations of pfhrp2-negative samples were low (< 0.001 ng/μL). These findings suggest that PfHRP2-based RDTs remain effective tools for malaria diagnosis in Nchelenge District, but further study is warranted to understand the potential for pfhrp2/3 deletions in southern Zambia where malaria transmission declined over the past decade.
UR - http://www.scopus.com/inward/record.url?scp=85064239173&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064239173&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.18-0859
DO - 10.4269/ajtmh.18-0859
M3 - Article
C2 - 30719965
AN - SCOPUS:85064239173
SN - 0002-9637
VL - 100
SP - 842
EP - 845
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 4
ER -