The scorpion toxin Bot IX is a potent member of the α-like family and has a unique N-terminal sequence extension

Marie France Martin-Eauclaire, Juan Salvatierra, Frank Bosmans, Pierre E. Bougis

Research output: Contribution to journalArticle

Abstract

We report the detailed chemical, immunological and pharmacological characterization of the α-toxin Bot IX from the Moroccan scorpion Buthus occitanus tunetanus venom. Bot IX, which consists of 70 amino acids, is a highly atypical toxin. It carries a unique N-terminal sequence extension and is highly lethal in mice. Voltage clamp recordings on oocytes expressing rat Nav1.2 or insect BgNav1 reveal that, similar to other α-like toxins, Bot IX inhibits fast inactivation of both variants. Moreover, Bot IX belongs to the same structural/immunological group as the α-like toxin Bot I. Remarkably, radioiodinated Bot IX competes efficiently with the classical α-toxin AaH II from Androctonus australis, and displays one of the highest affinities for Nav channels.

Original languageEnglish (US)
JournalFEBS Letters
DOIs
StateAccepted/In press - 2016

Keywords

  • Nav channel
  • Scorpion toxin
  • α-like toxin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Genetics
  • Cell Biology

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