Abstract
The immune system mediates tissue growth and homeostasis and is the first responder to injury or biomaterial implantation. Recently, it has been appreciated that immune cells play a critical role in wound healing and tissue repair and should thus be considered potentially beneficial, particularly in the context of scaffolds for regenerative medicine. In this study, we present a flow cytometric analysis of cellular recruitment to tissue-derived extracellular matrix scaffolds, where we quantitatively describe the infiltration and polarization of several immune subtypes, including macrophages, dendritic cells, neutrophils, monocytes, T cells, and B cells. We define a specific scaffold-associated macrophage (SAM) that expresses CD11b+F4/80+CD11c+/-CD206hiCD86+MHCII+ that are characteristic of an M2-like cell (CD206hi) with high antigen presentation capabilities (MHCII+). Adaptive immune cells tightly regulate the phenotype of a mature SAM. These studies provide a foundation for detailed characterization of the scaffold immune microenvironment of a given biomaterial scaffold to determine the effect of scaffold changes on immune response and subsequent therapeutic outcome of that material.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1044-1053 |
| Number of pages | 10 |
| Journal | Tissue Engineering - Part A |
| Volume | 23 |
| Issue number | 19-20 |
| DOIs | |
| State | Published - Oct 1 2017 |
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Keywords
- biomaterial implant
- extracellular matrix
- immune polarization
- macrophages
- T cells
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Biomaterials
- Biomedical Engineering
Cite this
The Scaffold Immune Microenvironment : Biomaterial-Mediated Immune Polarization in Traumatic and Nontraumatic Applications. / Sadtler, Kaitlyn; Allen, Brian W.; Estrellas, Kenneth; Housseau, Franck; Pardoll, Andrew Mark; Elisseeff, Jennifer Hartt.
In: Tissue Engineering - Part A, Vol. 23, No. 19-20, 01.10.2017, p. 1044-1053.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The Scaffold Immune Microenvironment
T2 - Biomaterial-Mediated Immune Polarization in Traumatic and Nontraumatic Applications
AU - Sadtler, Kaitlyn
AU - Allen, Brian W.
AU - Estrellas, Kenneth
AU - Housseau, Franck
AU - Pardoll, Andrew Mark
AU - Elisseeff, Jennifer Hartt
PY - 2017/10/1
Y1 - 2017/10/1
N2 - The immune system mediates tissue growth and homeostasis and is the first responder to injury or biomaterial implantation. Recently, it has been appreciated that immune cells play a critical role in wound healing and tissue repair and should thus be considered potentially beneficial, particularly in the context of scaffolds for regenerative medicine. In this study, we present a flow cytometric analysis of cellular recruitment to tissue-derived extracellular matrix scaffolds, where we quantitatively describe the infiltration and polarization of several immune subtypes, including macrophages, dendritic cells, neutrophils, monocytes, T cells, and B cells. We define a specific scaffold-associated macrophage (SAM) that expresses CD11b+F4/80+CD11c+/-CD206hiCD86+MHCII+ that are characteristic of an M2-like cell (CD206hi) with high antigen presentation capabilities (MHCII+). Adaptive immune cells tightly regulate the phenotype of a mature SAM. These studies provide a foundation for detailed characterization of the scaffold immune microenvironment of a given biomaterial scaffold to determine the effect of scaffold changes on immune response and subsequent therapeutic outcome of that material.
AB - The immune system mediates tissue growth and homeostasis and is the first responder to injury or biomaterial implantation. Recently, it has been appreciated that immune cells play a critical role in wound healing and tissue repair and should thus be considered potentially beneficial, particularly in the context of scaffolds for regenerative medicine. In this study, we present a flow cytometric analysis of cellular recruitment to tissue-derived extracellular matrix scaffolds, where we quantitatively describe the infiltration and polarization of several immune subtypes, including macrophages, dendritic cells, neutrophils, monocytes, T cells, and B cells. We define a specific scaffold-associated macrophage (SAM) that expresses CD11b+F4/80+CD11c+/-CD206hiCD86+MHCII+ that are characteristic of an M2-like cell (CD206hi) with high antigen presentation capabilities (MHCII+). Adaptive immune cells tightly regulate the phenotype of a mature SAM. These studies provide a foundation for detailed characterization of the scaffold immune microenvironment of a given biomaterial scaffold to determine the effect of scaffold changes on immune response and subsequent therapeutic outcome of that material.
KW - biomaterial implant
KW - extracellular matrix
KW - immune polarization
KW - macrophages
KW - T cells
UR - http://www.scopus.com/inward/record.url?scp=85032038136&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85032038136&partnerID=8YFLogxK
U2 - 10.1089/ten.tea.2016.0304
DO - 10.1089/ten.tea.2016.0304
M3 - Article
C2 - 27736323
AN - SCOPUS:85032038136
VL - 23
SP - 1044
EP - 1053
JO - Tissue Engineering - Part A.
JF - Tissue Engineering - Part A.
SN - 1937-3341
IS - 19-20
ER -