The SAGA/TREX-2 subunit Sus1 binds widely to transcribed genes and affects mRNA turnover globally

Varinia García-Molinero, José García-Martínez, Rohit Reja, Pedro Furió-Tarí, Oreto Antúnez, Vinesh Vinayachandran, Ana Conesa, B. Franklin Pugh, José E. Pérez-Ortín, Susana Rodríguez-Navarro

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Eukaryotic transcription is regulated through two complexes, the general transcription factor IID (TFIID) and the coactivator Spt-Ada-Gcn5 acetyltransferase (SAGA). Recent findings confirm that both TFIID and SAGA contribute to the synthesis of nearly all transcripts and are recruited genome-wide in yeast. However, how this broad recruitment confers selectivity under specific conditions remains an open question. Results: Here we find that the SAGA/TREX-2 subunit Sus1 associates with upstream regulatory regions of many yeast genes and that heat shock drastically changes Sus1 binding. While Sus1 binding to TFIID-dominated genes is not affected by temperature, its recruitment to SAGA-dominated genes and RP genes is significantly disturbed under heat shock, with Sus1 relocated to environmental stress-responsive genes in these conditions. Moreover, in contrast to recent results showing that SAGA deubiquitinating enzyme Ubp8 is dispensable for RNA synthesis, genomic run-on experiments demonstrate that Sus1 contributes to synthesis and stability of a wide range of transcripts. Conclusions: Our study provides support for a model in which SAGA/TREX-2 factor Sus1 acts as a global transcriptional regulator in yeast but has differential activity at yeast genes as a function of their transcription rate or during stress conditions.

Original languageEnglish (US)
Article number13
JournalEpigenetics and Chromatin
Volume11
Issue number1
DOIs
StatePublished - Mar 29 2018

Keywords

  • ChIP-exo
  • GRO
  • SAGA
  • Sus1
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'The SAGA/TREX-2 subunit Sus1 binds widely to transcribed genes and affects mRNA turnover globally'. Together they form a unique fingerprint.

Cite this