The saccharomyces cerevisiae SCRaMbLE system and genome minimization

Jessica Dymond, Jef Boeke

Research output: Contribution to journalArticle

Abstract

We have recently reported the first partially synthetic eukaryotic genome. Saccharomyces cerevisiae chromosomes synIXR and semi-synVIL are fully synthetic versions of the right arm of chromosome IX and the telomeric segment of the left arm of chromosome VI, respectively, and represent the beginning of the synthetic yeast genome project, Sc2.0, that progressively replaces native yeast DNA with synthetic sequences. We have designed synthetic chromosome sequences according to principles specifying a wild-type phenotype, highly stable genome, and maintenance of genetic flexibility. Although other synthetic genome projects exist, the Sc2.0 approach is unique in that we have implemented design specifications predicted to generate a wild-type phenotype until induction of "SCRaMbLE," an inducible evolution system that generates significant genetic diversity. Here we further explore the significance of Sc2.0 and show how SCRaMbLE can serve as a genome minimization tool.

Original languageEnglish (US)
Pages (from-to)168-171
Number of pages4
JournalBioengineered Bugs
Volume3
Issue number3
DOIs
StatePublished - Jul 2012

Fingerprint

Yeast
Saccharomyces cerevisiae
Chromosomes
Genes
Genome
Yeasts
Phenotype
DNA
Maintenance
Specifications

Keywords

  • Genome minimization
  • Sc2.0
  • Synthetic genome
  • synVIL
  • Yeast

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

The saccharomyces cerevisiae SCRaMbLE system and genome minimization. / Dymond, Jessica; Boeke, Jef.

In: Bioengineered Bugs, Vol. 3, No. 3, 07.2012, p. 168-171.

Research output: Contribution to journalArticle

Dymond, Jessica ; Boeke, Jef. / The saccharomyces cerevisiae SCRaMbLE system and genome minimization. In: Bioengineered Bugs. 2012 ; Vol. 3, No. 3. pp. 168-171.
@article{b51015854fe44613a4d6b442e6e23fe1,
title = "The saccharomyces cerevisiae SCRaMbLE system and genome minimization",
abstract = "We have recently reported the first partially synthetic eukaryotic genome. Saccharomyces cerevisiae chromosomes synIXR and semi-synVIL are fully synthetic versions of the right arm of chromosome IX and the telomeric segment of the left arm of chromosome VI, respectively, and represent the beginning of the synthetic yeast genome project, Sc2.0, that progressively replaces native yeast DNA with synthetic sequences. We have designed synthetic chromosome sequences according to principles specifying a wild-type phenotype, highly stable genome, and maintenance of genetic flexibility. Although other synthetic genome projects exist, the Sc2.0 approach is unique in that we have implemented design specifications predicted to generate a wild-type phenotype until induction of {"}SCRaMbLE,{"} an inducible evolution system that generates significant genetic diversity. Here we further explore the significance of Sc2.0 and show how SCRaMbLE can serve as a genome minimization tool.",
keywords = "Genome minimization, Sc2.0, Synthetic genome, synVIL, Yeast",
author = "Jessica Dymond and Jef Boeke",
year = "2012",
month = "7",
doi = "10.4161/bbug.19543",
language = "English (US)",
volume = "3",
pages = "168--171",
journal = "Bioengineered",
issn = "2165-5979",
publisher = "Taylor and Francis Ltd.",
number = "3",

}

TY - JOUR

T1 - The saccharomyces cerevisiae SCRaMbLE system and genome minimization

AU - Dymond, Jessica

AU - Boeke, Jef

PY - 2012/7

Y1 - 2012/7

N2 - We have recently reported the first partially synthetic eukaryotic genome. Saccharomyces cerevisiae chromosomes synIXR and semi-synVIL are fully synthetic versions of the right arm of chromosome IX and the telomeric segment of the left arm of chromosome VI, respectively, and represent the beginning of the synthetic yeast genome project, Sc2.0, that progressively replaces native yeast DNA with synthetic sequences. We have designed synthetic chromosome sequences according to principles specifying a wild-type phenotype, highly stable genome, and maintenance of genetic flexibility. Although other synthetic genome projects exist, the Sc2.0 approach is unique in that we have implemented design specifications predicted to generate a wild-type phenotype until induction of "SCRaMbLE," an inducible evolution system that generates significant genetic diversity. Here we further explore the significance of Sc2.0 and show how SCRaMbLE can serve as a genome minimization tool.

AB - We have recently reported the first partially synthetic eukaryotic genome. Saccharomyces cerevisiae chromosomes synIXR and semi-synVIL are fully synthetic versions of the right arm of chromosome IX and the telomeric segment of the left arm of chromosome VI, respectively, and represent the beginning of the synthetic yeast genome project, Sc2.0, that progressively replaces native yeast DNA with synthetic sequences. We have designed synthetic chromosome sequences according to principles specifying a wild-type phenotype, highly stable genome, and maintenance of genetic flexibility. Although other synthetic genome projects exist, the Sc2.0 approach is unique in that we have implemented design specifications predicted to generate a wild-type phenotype until induction of "SCRaMbLE," an inducible evolution system that generates significant genetic diversity. Here we further explore the significance of Sc2.0 and show how SCRaMbLE can serve as a genome minimization tool.

KW - Genome minimization

KW - Sc2.0

KW - Synthetic genome

KW - synVIL

KW - Yeast

UR - http://www.scopus.com/inward/record.url?scp=84862742295&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862742295&partnerID=8YFLogxK

U2 - 10.4161/bbug.19543

DO - 10.4161/bbug.19543

M3 - Article

VL - 3

SP - 168

EP - 171

JO - Bioengineered

JF - Bioengineered

SN - 2165-5979

IS - 3

ER -