The roles of complement receptor 3 and Fcγ receptors during Leishmania phagosome maturation

Rachel Polando, Upasna Gaur Dixit, Cristina R. Carter, Blake Jones, James P. Whitcom, Wibke Ballhorn, Melissa Harintho, Christopher L. Jerde, Mary E. Wilson, Mary Ann McDowell

Research output: Contribution to journalArticle

Abstract

Leishmania are intracellular parasites adapted to surviving in macrophages, whose primary function is elimination of invading pathogens. Leishmania entry into host cells is receptor-mediated. These parasites are able to engage multiple host cell-surface receptors, including MR, TLRs, CR3, and FcγRs. Here, we investigated the role of CR3 and FcγR engagement on the maturation of Leishmania-containing phagosomes using CD11b-/- and FcγR-/- macrophages, and assessing EEA1 and lysosome-associated proteins is necessary for the phagosome maturation delay, characteristic of Leishmania infection. Leishmania-containing phagosomes do not fuse with lyosomes until 5 h postinfection in WT mice. Phagolysosome fusion occurs by 1 h in CD11b and FcγR common chain KO macrophages, although receptor deficiency does not influence Leishmania entry or viability. We also investigated the influence of serum components and their effects on phagosome maturation progression. Opsonization with normal mouse serum, complement-deficient serum, or serum from Leishmania-infected mice all influenced phagosome maturation progression. Our results indicate that opsonophagocytosis influences phagosomal trafficking of Leishmania without altering the intracellular fate.

Original languageEnglish (US)
Pages (from-to)921-932
Number of pages12
JournalJournal of Leukocyte Biology
Volume93
Issue number6
DOIs
StatePublished - Jun 1 2013

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Keywords

  • CD11b
  • Deficient
  • EEA1
  • LAMP1
  • Mannose receptor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Cite this

Polando, R., Dixit, U. G., Carter, C. R., Jones, B., Whitcom, J. P., Ballhorn, W., Harintho, M., Jerde, C. L., Wilson, M. E., & McDowell, M. A. (2013). The roles of complement receptor 3 and Fcγ receptors during Leishmania phagosome maturation. Journal of Leukocyte Biology, 93(6), 921-932. https://doi.org/10.1189/jlb.0212086