The role of the spleen in laparoscopy-associated inflammatory response

S. L. Bachman, E. J. Hanly, D. Saad, J. I. Nwanko, J. Lamb, A. E. Herring, Michael Marohn, A. De-Maio, M. A. Talamini

Research output: Contribution to journalArticle

Abstract

Background: Carbon dioxide (CO2) pneumoperitoneum alters the inflammatory response in animal models of sepsis. The spleen is a key organ in inflammation and its removal was predicted to modify this effect. Methods: The acute phase inflammatory response to lipopolysaccharide (LPS) challenge in male rats was examined for the effects of splenectomy (spx) and the technique of removal (open or laparpscopic). A series of experiments compared LPS-only controls with LPS injection 2 or 9 days following open spx, lap CO2 spx, open sham, or lap CO2 sham. The method of splenectomy was studied by randomization to control, open spx, lap CO2 spx, lap helium (He) spx, or lap air spx with LPS challenge on postoperative day 2. Serum levels of tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ) and, interleutin (IL) 10 were collected at multiple time points, assayed by commercial enzyme-linked immunosorbent assay, analyzed by analysis of variance. Results: Levels of TNF-α at 1.5 were significantly lower following open sham than following lap sham (p <0.05). Splenectomy drastically reduced INF-γ and TNF-α levels compared to controls (p <0.05) on postoperative day 2. No method of spx preserved TNF-α or INF-γ responses. Recovery of TNF-α response on day 9 was delayed in the spx groups. Conclusions: Splenectomy dramatically reduces TNF-α and INF-γ responses to LPS challenge, although by different mechanisms. Pneumoperitoneum-mediated modulation of the septic inflammatory response is partially dependent on the spleen.

Original languageEnglish (US)
Pages (from-to)1035-1044
Number of pages10
JournalSurgical Endoscopy and Other Interventional Techniques
Volume19
Issue number8
DOIs
StatePublished - Aug 2005
Externally publishedYes

Fingerprint

Laparoscopy
Spleen
Tumor Necrosis Factor-alpha
Lipopolysaccharides
Splenectomy
Interferons
Pneumoperitoneum
Helium
Acute-Phase Reaction
Random Allocation
Carbon Dioxide
Sepsis
Analysis of Variance
Animal Models
Enzyme-Linked Immunosorbent Assay
Air
Inflammation
Injections
Serum

Keywords

  • Endotoxemia
  • Inflammatory
  • Laparoscopy
  • Lipopolysaccharide
  • Pneumoperitoneum
  • response
  • Splenectomy

ASJC Scopus subject areas

  • Surgery

Cite this

Bachman, S. L., Hanly, E. J., Saad, D., Nwanko, J. I., Lamb, J., Herring, A. E., ... Talamini, M. A. (2005). The role of the spleen in laparoscopy-associated inflammatory response. Surgical Endoscopy and Other Interventional Techniques, 19(8), 1035-1044. https://doi.org/10.1007/s00464-004-8820-2

The role of the spleen in laparoscopy-associated inflammatory response. / Bachman, S. L.; Hanly, E. J.; Saad, D.; Nwanko, J. I.; Lamb, J.; Herring, A. E.; Marohn, Michael; De-Maio, A.; Talamini, M. A.

In: Surgical Endoscopy and Other Interventional Techniques, Vol. 19, No. 8, 08.2005, p. 1035-1044.

Research output: Contribution to journalArticle

Bachman, SL, Hanly, EJ, Saad, D, Nwanko, JI, Lamb, J, Herring, AE, Marohn, M, De-Maio, A & Talamini, MA 2005, 'The role of the spleen in laparoscopy-associated inflammatory response', Surgical Endoscopy and Other Interventional Techniques, vol. 19, no. 8, pp. 1035-1044. https://doi.org/10.1007/s00464-004-8820-2
Bachman, S. L. ; Hanly, E. J. ; Saad, D. ; Nwanko, J. I. ; Lamb, J. ; Herring, A. E. ; Marohn, Michael ; De-Maio, A. ; Talamini, M. A. / The role of the spleen in laparoscopy-associated inflammatory response. In: Surgical Endoscopy and Other Interventional Techniques. 2005 ; Vol. 19, No. 8. pp. 1035-1044.
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abstract = "Background: Carbon dioxide (CO2) pneumoperitoneum alters the inflammatory response in animal models of sepsis. The spleen is a key organ in inflammation and its removal was predicted to modify this effect. Methods: The acute phase inflammatory response to lipopolysaccharide (LPS) challenge in male rats was examined for the effects of splenectomy (spx) and the technique of removal (open or laparpscopic). A series of experiments compared LPS-only controls with LPS injection 2 or 9 days following open spx, lap CO2 spx, open sham, or lap CO2 sham. The method of splenectomy was studied by randomization to control, open spx, lap CO2 spx, lap helium (He) spx, or lap air spx with LPS challenge on postoperative day 2. Serum levels of tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ) and, interleutin (IL) 10 were collected at multiple time points, assayed by commercial enzyme-linked immunosorbent assay, analyzed by analysis of variance. Results: Levels of TNF-α at 1.5 were significantly lower following open sham than following lap sham (p <0.05). Splenectomy drastically reduced INF-γ and TNF-α levels compared to controls (p <0.05) on postoperative day 2. No method of spx preserved TNF-α or INF-γ responses. Recovery of TNF-α response on day 9 was delayed in the spx groups. Conclusions: Splenectomy dramatically reduces TNF-α and INF-γ responses to LPS challenge, although by different mechanisms. Pneumoperitoneum-mediated modulation of the septic inflammatory response is partially dependent on the spleen.",
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T1 - The role of the spleen in laparoscopy-associated inflammatory response

AU - Bachman, S. L.

AU - Hanly, E. J.

AU - Saad, D.

AU - Nwanko, J. I.

AU - Lamb, J.

AU - Herring, A. E.

AU - Marohn, Michael

AU - De-Maio, A.

AU - Talamini, M. A.

PY - 2005/8

Y1 - 2005/8

N2 - Background: Carbon dioxide (CO2) pneumoperitoneum alters the inflammatory response in animal models of sepsis. The spleen is a key organ in inflammation and its removal was predicted to modify this effect. Methods: The acute phase inflammatory response to lipopolysaccharide (LPS) challenge in male rats was examined for the effects of splenectomy (spx) and the technique of removal (open or laparpscopic). A series of experiments compared LPS-only controls with LPS injection 2 or 9 days following open spx, lap CO2 spx, open sham, or lap CO2 sham. The method of splenectomy was studied by randomization to control, open spx, lap CO2 spx, lap helium (He) spx, or lap air spx with LPS challenge on postoperative day 2. Serum levels of tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ) and, interleutin (IL) 10 were collected at multiple time points, assayed by commercial enzyme-linked immunosorbent assay, analyzed by analysis of variance. Results: Levels of TNF-α at 1.5 were significantly lower following open sham than following lap sham (p <0.05). Splenectomy drastically reduced INF-γ and TNF-α levels compared to controls (p <0.05) on postoperative day 2. No method of spx preserved TNF-α or INF-γ responses. Recovery of TNF-α response on day 9 was delayed in the spx groups. Conclusions: Splenectomy dramatically reduces TNF-α and INF-γ responses to LPS challenge, although by different mechanisms. Pneumoperitoneum-mediated modulation of the septic inflammatory response is partially dependent on the spleen.

AB - Background: Carbon dioxide (CO2) pneumoperitoneum alters the inflammatory response in animal models of sepsis. The spleen is a key organ in inflammation and its removal was predicted to modify this effect. Methods: The acute phase inflammatory response to lipopolysaccharide (LPS) challenge in male rats was examined for the effects of splenectomy (spx) and the technique of removal (open or laparpscopic). A series of experiments compared LPS-only controls with LPS injection 2 or 9 days following open spx, lap CO2 spx, open sham, or lap CO2 sham. The method of splenectomy was studied by randomization to control, open spx, lap CO2 spx, lap helium (He) spx, or lap air spx with LPS challenge on postoperative day 2. Serum levels of tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ) and, interleutin (IL) 10 were collected at multiple time points, assayed by commercial enzyme-linked immunosorbent assay, analyzed by analysis of variance. Results: Levels of TNF-α at 1.5 were significantly lower following open sham than following lap sham (p <0.05). Splenectomy drastically reduced INF-γ and TNF-α levels compared to controls (p <0.05) on postoperative day 2. No method of spx preserved TNF-α or INF-γ responses. Recovery of TNF-α response on day 9 was delayed in the spx groups. Conclusions: Splenectomy dramatically reduces TNF-α and INF-γ responses to LPS challenge, although by different mechanisms. Pneumoperitoneum-mediated modulation of the septic inflammatory response is partially dependent on the spleen.

KW - Endotoxemia

KW - Inflammatory

KW - Laparoscopy

KW - Lipopolysaccharide

KW - Pneumoperitoneum

KW - response

KW - Splenectomy

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