TY - JOUR
T1 - The role of soluble interleukin-4 receptor and interleukin-5 antibody in preventing late-phase allergy-induced eustachian tube dysfunction
AU - Pollock, Hoke W.
AU - Ebert, Charles S.
AU - Dubin, Marc G.
AU - White, David R.
AU - Prazma, Jiri
AU - Pillsbury, Harold C.
N1 - Funding Information:
From the Department of Surgery, Division of Otolaryngolo-gy/HNS, University of North Carolina Hospitals. Funded in part by a resident research grant from the American Academy of Otolaryngic Allergy (2000-2001). Portions were presented at the Annual Meeting of the Amer-ican Academy of Otolaryngic Allergy, September 2000. Reprint requests: Jiri Prazma, MD, PhD, Department of Sur-gery, Division of Otolaryngology/HNS, CB#7070, Bur-nett-Womack Clinical Sciences Building, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7070; email, hpollock@med.unc.edu. Copyright © 2002 by the American Academy of Otolaryn-gology–Head and Neck Surgery Foundation, Inc. 0194-5998/2002/$35.00 + 0 23/1/126901 doi:10.1067/mhn.2002.126901
PY - 2002/9
Y1 - 2002/9
N2 - OBJECTIVES: We investigated the role of soluble interleukin (IL)-4 receptors (slL-4R) and IL-5 antibodies (IL-5Ab) in preventing allergic eustachian tube dysfunction (ETD) and middle ear effusion (MEE). STUDY DESIGN: Brown-Norway rats were sensitized to ovalbumin (OVA) and challenged transtympanically. Two groups of rats received either IL-4R or IL-5Ab transtympanically 1 hour before challenge. Three additional groups were used as controls. Following the second transtympanic challenge, the ventilatory and clearance functions of the eustachian tube (ET) were assessed at 0, 2, and 8 hours. Histology was prepared using cut paraffin sections stained with hematoxylin and eosin. RESULTS: sIL-4R-pretreated rats showed no significant changes in ventilatory or clearance functions of the ET or inflammatory changes in ET mucosa, whereas IL-5Ab pretreatment showed significant late ventilatory and clearance dysfunction as well as inflammatory mucosal changes. CONCLUSION: These data demonstrate that the late-phase allergic inflammatory response that leads to subsequent formation of ETD and MEE is prevented by pretreatment with sIL-4R and, more modestly, with IL-5Ab.
AB - OBJECTIVES: We investigated the role of soluble interleukin (IL)-4 receptors (slL-4R) and IL-5 antibodies (IL-5Ab) in preventing allergic eustachian tube dysfunction (ETD) and middle ear effusion (MEE). STUDY DESIGN: Brown-Norway rats were sensitized to ovalbumin (OVA) and challenged transtympanically. Two groups of rats received either IL-4R or IL-5Ab transtympanically 1 hour before challenge. Three additional groups were used as controls. Following the second transtympanic challenge, the ventilatory and clearance functions of the eustachian tube (ET) were assessed at 0, 2, and 8 hours. Histology was prepared using cut paraffin sections stained with hematoxylin and eosin. RESULTS: sIL-4R-pretreated rats showed no significant changes in ventilatory or clearance functions of the ET or inflammatory changes in ET mucosa, whereas IL-5Ab pretreatment showed significant late ventilatory and clearance dysfunction as well as inflammatory mucosal changes. CONCLUSION: These data demonstrate that the late-phase allergic inflammatory response that leads to subsequent formation of ETD and MEE is prevented by pretreatment with sIL-4R and, more modestly, with IL-5Ab.
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U2 - 10.1067/mhn.2002.126901
DO - 10.1067/mhn.2002.126901
M3 - Article
C2 - 12297806
AN - SCOPUS:0036740617
SN - 0194-5998
VL - 127
SP - 169
EP - 176
JO - Otolaryngology - Head and Neck Surgery
JF - Otolaryngology - Head and Neck Surgery
IS - 3
ER -