The role of smooth muscle cell differentiation in the mechanism of obliteration of processus vaginalis

Munevver Hosgor, Irfan Karaca, Erdener Ozer, Gulsun Erdag, Cagnur Ulukus, Orhan Fescekoglu, Masanori Aikawa

Research output: Contribution to journalArticlepeer-review

Abstract

Background/Purpose Development of indirect inguinal hernia and hydrocele in childhood is readily explained by the persistence of smooth muscle component around the processus vaginalis (PV) after the descent of the testis into the scrotum. The aim of this study was to investigate the expression of smooth muscle myosin heavy chain (SM MHC) isoforms as the markers of smooth muscle cell (SMC) differentiation in childhood inguinal hernia and hydrocele and in age-matched controls. Methods The authors analyzed sacs from patients with inguinal hernia (male, 10; female, 10) and hydrocele (n = 10) immunohistochemically using monoclonal antibodies against α-smooth muscle actin, SM1, SM2 and SMemb. Peritoneal samples (male, 5; female, 5) obtained from age-matched patients served as controls. Immunostaining was evaluated with semiquantitative scoring and χ2 test. Results The expression pattern of SM MHC isoforms did not differ among sacs obtained from female inguinal hernia when compared with that of controls. However, strong expression of SMemb within the sac walls of male inguinal hernia and SM1 in hydrocele groups were observed. Conclusions Our results indicate that SMC differentiation may play an important role in the obliteration of processus vaginalis in male inguinal hernia and hydrocele after the descent of the testis.

Original languageEnglish (US)
Pages (from-to)1018-1023
Number of pages6
JournalJournal of pediatric surgery
Volume39
Issue number7
DOIs
StatePublished - Jul 1 2004

Keywords

  • Myosin heavy chains
  • hydrocele
  • inguinal hernia
  • smooth muscle

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health

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